Takikita Mikiko, Chung Joon-Yong, Hewitt Stephen M
Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, TARP Lab, MSC 4605, Bethesda, MD 20892-4605, USA.
Curr Opin Biotechnol. 2007 Aug;18(4):318-25. doi: 10.1016/j.copbio.2007.05.007. Epub 2007 Jul 20.
The tissue microarray has enabled high-throughput pathology. Rather than the laborious review of individual slides and issues of assay reproducibility across large series of specimens, tissue microarrays allow the review of a single stain on a single slide containing tens to hundreds of samples. This is a paradigm shift in pathology, away from histomorphology and toward molecular characterization by immunohistochemistry. This platform allows large retrospective clinical studies of biomarkers for correlation with outcome and can equally well be applied toward high-throughput analysis of cell lines and xenografts. Tissue microarrays encourage novel approaches to assaying tissue with retained histomorphology and have enabled image analysis in pathology. The reduction of tissue to an analyte for high-throughput analysis has highlighted the importance of a high quality tissue and the impact of tissue handling and processing in the quality of data that can be obtained from analysis of tissue.
组织微阵列实现了高通量病理学。与费力地逐个审查玻片以及大量标本间检测重复性的问题不同，组织微阵列允许在一张载有数十至数百个样本的玻片上对单一染色进行审查。这是病理学领域的范式转变，从组织形态学转向通过免疫组织化学进行分子特征分析。该平台可用于对生物标志物进行大规模回顾性临床研究以与结果相关联，同样也能很好地应用于细胞系和异种移植的高通量分析。组织微阵列鼓励采用保留组织形态学的新型组织检测方法，并实现了病理学中的图像分析。将组织简化为用于高通量分析的分析物，凸显了高质量组织的重要性以及组织处理和加工对从组织分析中获得的数据质量的影响。
