Famulari N G, Fleissner E
J Virol. 1976 Feb;17(2):605-13. doi: 10.1128/JVI.17.2.605-613.1976.
The synthesis of the 50S genomic RNA and strucural proteins of Sendai virus was examined with respect to their utilization in virus assembly. It was found that during a single cycle of infection, 50S RNA was synthesized before the structural proteins and that both RNA and protein were synthesized 2 to 4 h before their appearance in released virions. Pulse-chase labeling indicated that the NP and P proteins synthesized early and the M and F proteins synthesized late were preferentially incorporated into virus relative to the other viral proteins. The kinetics of incorporation of pulse-labeled NP protein suggested that it was withdrawn from a relatively large pool whereas the M protein appeared to be present in a relatively small pool in the cytoplasm. Further, it was possible to chase pulse-labeled M protein, but not NP protein, from the cell during an 8-h time period.
就仙台病毒50S基因组RNA和结构蛋白在病毒组装中的利用情况进行了研究。结果发现，在单个感染周期中，50S RNA在结构蛋白之前合成，并且RNA和蛋白质在其出现在释放的病毒粒子之前2至4小时合成。脉冲追踪标记表明，相对于其他病毒蛋白，早期合成的NP和P蛋白以及晚期合成的M和F蛋白优先掺入病毒中。脉冲标记的NP蛋白掺入动力学表明，它是从相对较大的池中被提取出来的，而M蛋白似乎在细胞质中以相对较小的池存在。此外，在8小时的时间段内，可以从细胞中追踪脉冲标记的M蛋白，但不能追踪NP蛋白。
