用辣根过氧化物酶标记的抗体片段对猴病毒40 T抗原进行免疫细胞化学定位。
Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.
作者信息
Tabuchi K, Lehman J M, Kirsch W M
出版信息
J Virol. 1976 Feb;17(2):668-71. doi: 10.1128/JVI.17.2.668-671.1976.
Abstract
T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a synthesis of T antigen on cytoplasmic ribosomes, with subsequent transfer to the nucleus.
摘要
通过Fab'抗体缀合物在超微结构水平上对T抗原进行定位，发现其存在于猴病毒40裂解感染和转化的细胞中。这种病毒特异性蛋白在感染后3小时就最早出现在允许性细胞的细胞质中。在随后的时间间隔内，T抗原定位于细胞质和细胞核中，最终（24小时）定位于细胞核中。这些结果表明T抗原在细胞质核糖体上合成，随后转移至细胞核。
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