来自经猿猴病毒40温度敏感突变体转化的细胞的热不稳定T（肿瘤）抗原。
Thermolabile T (tumor) antigen from cells transformed by a temperature-sensitive mutant of simian virus 40.
作者信息
Tenen D G, Baygell P, Livingston D M
出版信息
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4351-5. doi: 10.1073/pnas.72.11.4351.
Abstract
Partially purified tumor (T) antigen from a strain of Chinese hamster lung cells transformed by wild-type simian virus 40 (SV 40) and either of two temperature-sensitive SV 40 mutants has been studied as a DNA binding protein. The DNA binding activity present in the T-antigen-containing fractions is inhibited by purified hamster anti-T IgG but not by equivalent amounts of nonimmune hamster IgG. T from either wild-type- or tsC219-transformed cells is relatively stable during heating at 44 degrees compared to T prepared from tsA239-transformed cells. These results strongly suggest that T is a product of the SV 40 A gene.
摘要
从一株经野生型猿猴病毒40（SV 40）和两种温度敏感型SV 40突变体之一转化的中国仓鼠肺细胞中部分纯化的肿瘤（T）抗原，已作为一种DNA结合蛋白进行了研究。含T抗原的组分中存在的DNA结合活性被纯化的仓鼠抗T IgG抑制，但等量的非免疫仓鼠IgG则无此作用。与从tsA239转化细胞制备的T相比，野生型或tsC219转化细胞中的T在44摄氏度加热时相对稳定。这些结果有力地表明T是SV 40 A基因的产物。
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