J Virol. 1976 Mar;17(3):692-704. doi: 10.1128/JVI.17.3.692-704.1976.
Polyoma (Py) virus multiplies, at 34 and 38.5 C, in wild-type (WT-4) and in ts A1S9 mouse L cells, which are temperature sensitive for growth and for DNA replication (R. Sheinin, 1976; L. H. Thompson et al., 1970). De novo synthesis of double-stranded, fully covalently closed Py DNA has been shown to proceed by semiconservative replication in WT-4 and ts A1S9 cells at the permissive and nonpermissive temperatures. Cell DNA is made late during infection, by both cell types and at both temperatures. Semiconservative replication of cell DNA proceeds in Py-infected WT-4 cells incubated at 34 or at 38.5 C and in Py-infected ts A1S9 cells incubated at 34 C. In virus-infected ts A1S9 cells incubated at 38.5 C, cell DNA synthesis appears to proceed almost entirely by a process analogous to repair replication. The inability of ts A1S9 cells to produce large-molecular-weight chromosomal DNA strands, at 38.5 C, by the normal mechanism is not overcome by Py infection.
多瘤（Py）病毒在34℃和38.5℃时，能在野生型（WT-4）和ts A1S9小鼠L细胞中繁殖，这两种细胞对生长和DNA复制具有温度敏感性（R. 谢宁，1976；L. H. 汤普森等人，1970）。已证明双链、完全共价闭合的Py DNA的从头合成在允许温度和非允许温度下，在WT-4和ts A1S9细胞中通过半保留复制进行。细胞DNA在感染后期由两种细胞类型在两种温度下合成。细胞DNA的半保留复制在34℃或38.5℃培养的Py感染的WT-4细胞以及34℃培养的Py感染的ts A1S9细胞中进行。在38.5℃培养的病毒感染的ts A1S9细胞中，细胞DNA合成似乎几乎完全通过类似于修复复制的过程进行。ts A1S9细胞在38.5℃时无法通过正常机制产生大分子染色体DNA链的情况，不会因Py感染而得到克服。
