Lee Sang-Eun, Kim Heung-Chul, Chong Sung-Tae, Klein Terry A, Lee Won-Ja
Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Republic of Korea.
Vet Parasitol. 2007 Sep 1;148(2):149-55. doi: 10.1016/j.vetpar.2007.04.015. Epub 2007 Jul 17.
Adult mosquito collections using New Jersey light traps and Black-hole light traps were conducted to determine the potential vectors and the relative mosquito infection rates of Dirofilaria immitis and Dirofilaria repens in Gyeonggi and Gangwon Provinces, Republic of Korea, 2005. Dirofilaria spp. were confirmed by polymerase chain reaction (PCR) using species-specific primers for D. immitis and D. repens. Minimum field infection rates (MFIR) [MFIR = (number infected pools/total number of mosquitoes) x 1000] of 12 pools/2059 total number mosquitoes (5.8) and 21 pools (10.1) of the wild caught mosquitoes were positive by PCR for D. immitis and D. repens, respectively. Dual infections of both D. immitis and D. repens were detected by PCR in eight pools (3.9). Anopheles sinensis sensu lato (includes An. sinensis s.s., An. pullus, An. kleini, An. belenrae, and An. lesteri), An. sineroides, Aedes vexans nipponi, Culex pipiens and Armigeres subalbatus were found to be infected and are potential vectors of D. immitis and D. repens in Korea. Infections observed in mosquitoes represent potential health risks for domestic animal and human populations.
2005年，在韩国京畿道和江原道，使用新泽西诱蚊灯和黑洞诱蚊灯收集成年蚊子，以确定犬恶丝虫和匐行恶丝虫的潜在病媒及相对蚊子感染率。使用针对犬恶丝虫和匐行恶丝虫的种特异性引物，通过聚合酶链反应（PCR）确认恶丝虫属。野生捕获蚊子中，犬恶丝虫和匐行恶丝虫的最小田间感染率（MFIR）[MFIR =（感染池数量/蚊子总数）×1000]分别为12池/2059只蚊子总数（5.8）和21池（10.1），经PCR检测呈阳性。通过PCR在8个池（3.9）中检测到犬恶丝虫和匐行恶丝虫的双重感染。发现中华按蚊复合组（包括中华按蚊指名亚种、棕毛按蚊、克氏按蚊、贝氏按蚊和雷氏按蚊）、拟中华按蚊、骚扰伊蚊日本亚种、致倦库蚊和白纹伊蚊感染，它们是韩国犬恶丝虫和匐行恶丝虫的潜在病媒。在蚊子中观察到的感染对家畜和人类群体构成潜在健康风险。
