Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Force Health Protection and Preventive Medicine, MEDDAC-Korea/65th Medical Brigade, Unit 15281, APO AP 96271-5281, USA.
Viruses. 2019 Mar 5;11(3):222. doi: 10.3390/v11030222.
Arboviruses continue to be a significant global health concern. The unbiased metagenomic analyses of mosquito-borne and mosquito-specific viruses are useful to understand viral diversity and for the surveillance of pathogens of medical and veterinary importance. Metagenomic analysis was conducted on 6368 mosquitoes (736 pools), covering 16 species from 18 locations throughout the Republic of Korea (ROK) in 2016. In this report, we describe three viruses detected in a single pool of collected at Yongsan U.S. Army Garrison, located in a densely populated district of Seoul, the ROK. The three novel viruses, designated as Yongsan bunyavirus 1 (YBV1), Yongsan picorna-like virus 3 (YPLV3) and Yongsan sobemo-like virus 1 (YSLV1), share sequence and structural characteristics with members belonging to the family , order , and family , with shared RNA-dependent RNA polymerase (RdRp) amino acid identities of 40%, 42% and 86%, respectively. The real-time reverse transcription and polymerase chain reaction (RT-PCR) of 3493 (257 pools) showed a high prevalence of YBV1 and YSLV1 viruses, which were present in 65% and 62% of tested pools, respectively. This study highlighted the utility of a metagenomic sequencing approach for arbovirus discovery and for a better understanding of the virome of potential medically relevant vectors.
虫媒病毒继续是一个重大的全球健康问题。对蚊虫传播和蚊虫特异性病毒进行无偏倚的宏基因组分析有助于了解病毒多样性,并监测具有医学和兽医重要性的病原体。2016 年,对来自韩国(ROK)18 个地点的 16 个物种的 6368 只蚊子(736 个蚊群)进行了宏基因组分析。在本报告中,我们描述了在位于韩国首尔人口稠密区的龙山美军基地采集的一个蚊群中发现的三种病毒。这三种新病毒分别命名为龙山布尼亚病毒 1(YBV1)、龙山微小 RNA 病毒 3(YPLV3)和龙山 sobemo 样病毒 1(YSLV1),与属于家族、目和家族的成员具有序列和结构特征,其 RNA 依赖性 RNA 聚合酶(RdRp)氨基酸同一性分别为 40%、42%和 86%。对 3493 只(257 个蚊群)进行的实时逆转录聚合酶链反应(RT-PCR)显示 YBV1 和 YSLV1 病毒的高流行率,分别存在于 65%和 62%的测试蚊群中。本研究强调了宏基因组测序方法在虫媒病毒发现和更好地了解潜在具有医学相关性的媒介病毒组方面的应用。