Callejas S, Alberio R, Cabodevila J, Aller J, Catalano R, Teruel M, Dulout F
Area de Reproducción (FISFARVET), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Campus Universitario, Paraje Arroyo Seco S/N, B7000GHG Tandil, Buenos Aires, Argentina.
Anim Reprod Sci. 2008 Aug;107(1-2):9-19. doi: 10.1016/j.anireprosci.2007.06.014. Epub 2007 Jun 17.
To evaluate ovarian response in Angus cows previously treated with progesterone (P4), animals were randomly assigned to two groups: T600 group (n=14), 600 mg of P4/day. P4 was injected from days 3 to 7 of the estrous cycle. On day 7, superovulatory treatments began. The control group (n=12) was given vehicle only. The superovulatory treatments in the control group began on days 7-9 of the estrous cycle. The superovulatory total treatment dose of 400mg NIH FSH P1 was given twice a day over a 4-day period. Ultrasonography of the ovaries was conducted 3 days preceding the initiation of superovulatory treatment, every 24h. In both groups, an additional ultrasonographic evaluation was made at 24h after the end of superovulatory treatment. Blood samples were collected 4 days preceding the initiation of superovulatory treatment, every 24h. Additional samples were taken from the P600 group for 12 day after of initiation of superovulatory treatment every 24h, except on the fifth day after the initiation of superovulatory treatment. In the P600 group, P4 concentrations were greater than in the control group (P<0.01) and remained over 1 ng/ml up to day 11 after beginning of superovulatory treatment. The diameter of the dominant follicle was larger in the animals of the control group (P<0.01). Cows of the P600 group had a greater number of Class I (3-4mm) follicles (P<0.01). A significant day and treatment effect (P<0.01) were observed in Class II (5-9 mm) follicles. Effects due to treatment on the number of Class III follicles (P<0.05) were observed. In the P600 group, no estrous post-superovulatory was observed and there were no ovulations that occurred. Conversely, 100% of the cows of the control group showed estrous. In the P600 group, there were a greater number of Class III follicles (P<0.01) and a lesser number of Class II follicles (P<0.05) at 24h after the end of superovulatory. In the control group, 66.7% of the cows responded to superovulatory treatments. In conclusion, the daily administration of 600 mg of P4, from days 3 to 7 of the estrous cycle, produces an increase of plasma concentrations of this hormone from day 4, resulting in changes in follicular dynamics (absence of follicles greater than 10mm of diameter and an increase of the population of Class I follicles). As to the ovarian stimulation using Folltropin V in animals receiving a daily injection of 600 mg of P4 from days 3 to 7 of the estrous cycle, a greater population of follicles>or=10mm developed by 24h after superovulatory treatments were completed.
为评估先前用孕酮（P4）处理过的安格斯母牛的卵巢反应，将动物随机分为两组：T600组（n = 14），每天注射600 mg P4。在发情周期的第3至7天注射P4。在第7天开始进行超排处理。对照组（n = 12）仅给予赋形剂。对照组的超排处理在发情周期的第7 - 9天开始。超排总处理剂量为400mg NIH FSH P1，在4天内每天分两次给药。在开始超排处理前3天，每24小时对卵巢进行一次超声检查。两组在超排处理结束后24小时均进行了额外的超声评估。在开始超排处理前4天，每24小时采集一次血样。在超排处理开始后12天内，除超排处理开始后第5天外，每24小时从P600组采集额外的血样。在P600组中，P4浓度高于对照组（P < 0.01），并且在超排处理开始后直至第11天一直保持在1 ng/ml以上。对照组动物的优势卵泡直径更大（P < 0.01）。P600组的母牛有更多I类（3 - 4mm）卵泡（P < 0.01）。在II类（5 - 9mm）卵泡中观察到显著的天数和处理效应（P < 0.01）。观察到处理对III类卵泡数量的影响（P < 0.05）。在P600组中，超排后未观察到发情，也未发生排卵。相反，对照组100%的母牛表现出发情。在超排处理结束后24小时，P600组有更多的III类卵泡（P < 0.01）和更少的II类卵泡（P < 0.05）。在对照组中，66.7%的母牛对超排处理有反应。总之，在发情周期的第3至7天每天给予600 mg P4，从第4天起该激素的血浆浓度升高，导致卵泡动态变化（直径大于10mm的卵泡缺失，I类卵泡数量增加）。对于在发情周期的第3至7天每天注射600 mg P4的动物使用Folltropin V进行卵巢刺激，在超排处理完成后24小时，发育出更多直径≥10mm的卵泡。
