Nelson Shawn S, Glocka Padden P, Agarwal Sarika, Grimm David P, McBride Mark J
Department of Biological Sciences, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211, USA.
J Bacteriol. 2007 Oct;189(19):7145-50. doi: 10.1128/JB.00892-07. Epub 2007 Jul 20.
Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.
约翰逊氏黄杆菌细胞通过未知机制在表面快速滑行。转座子诱导的sprA突变体在琼脂上形成不扩散的菌落，在湿载片中观察的细胞在附着于表面方面存在缺陷，并且几乎完全不运动。完整细胞暴露于蛋白酶K会将270 kDa的SprA切割成几个大的肽段，这表明它部分暴露于细胞表面。
