Zhao Chengbiao, Liu Wei, Ling Hongli, Lu Shengxin, Zhang Yulan, Liu Jinting, Xi Rimo
The School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, People's Republic of China.
J Agric Food Chem. 2007 Aug 22;55(17):6879-84. doi: 10.1021/jf070978g. Epub 2007 Jul 24.
A polyclonal anti-gatifloxacin antibody has been prepared, and an indirect competitive enzyme-linked immunosorbent assay (cELISA) was developed on the basis of the antibody prepared for the first time. The antibody shows high sensitivity with an IC50 value of 2.6 ppb and excellent specificity with only a minor cross-reaction with lomefloxacin (3.0%) among common (fluoro)quinolones evaluated in this study. The high specificity of the antibody was explained by the molecular structures of related drugs by comparison with published research. The cELISA test kit developed has a detection limit of 0.05 ppb and could be used as a screening method to detect and regulate illegal use of gatifloxacin in food and food products. The test kit was applied to the detection of milk samples spiked by gatifloxacin. The recovery rates were in the range of 86-106%, whereas the intra- and interassay coefficients of variation were <14.3 and <19.6%, respectively.
已制备出一种多克隆抗加替沙星抗体，并基于首次制备的该抗体开发了一种间接竞争酶联免疫吸附测定法（cELISA）。该抗体显示出高灵敏度，IC50值为2.6 ppb，特异性良好，在本研究评估的常见（氟）喹诺酮类药物中，仅与洛美沙星有轻微交叉反应（3.0%）。通过与已发表的研究比较相关药物的分子结构，解释了该抗体的高特异性。所开发的cELISA检测试剂盒的检测限为0.05 ppb，可作为一种筛查方法，用于检测和监管食品及食品产品中加替沙星的非法使用情况。该检测试剂盒应用于加替沙星加标牛奶样品的检测。回收率在86 - 106%范围内，而批内和批间变异系数分别<14.3%和<19.6%。
