Yoshioka H
Japan Research Center of W.R. Grace & Co.-Conn., Kanagawa, Japan.
Biomaterials. 1991 Nov;12(9):861-4. doi: 10.1016/0142-9612(91)90075-l.
With a view to prevention of liposome aggregation in plasma which is a lethal problem when using artificial erythrocytes, modification of the liposome surface with polyethylene glycol (PEG)-bound cholesterol or phospholipid was studied. The cholesterol or phospholipid moiety works as a scaffold for immobilization of the modifiers to the liposome. The modified liposome aggregability is significantly reduced with increased PEG content and particularly with increased PEG chain length. In addition, the zeta potential of modified liposomes decreases with increased PEG chain length. It is concluded that the long PEG chain exposed on the liposome surface prevents the adsorption of plasma proteins on to the surface by its excluded volume effect and, as a result, effectively reduces the liposome aggregability in plasma.
鉴于在使用人工红细胞时，血浆中脂质体聚集是一个致命问题，因此研究了用聚乙二醇（PEG）结合的胆固醇或磷脂对脂质体表面进行修饰。胆固醇或磷脂部分作为将修饰剂固定到脂质体上的支架。随着PEG含量的增加，特别是PEG链长度的增加，修饰后的脂质体聚集性显著降低。此外，修饰脂质体的zeta电位随着PEG链长度的增加而降低。得出的结论是，暴露在脂质体表面的长PEG链通过其排阻体积效应阻止血浆蛋白吸附到表面，从而有效降低脂质体在血浆中的聚集性。
