Chiel E, Gottlieb Y, Zchori-Fein E, Mozes-Daube N, Katzir N, Inbar M, Ghanim M
Department of Entomology, Newe-Ya'ar Research Center, ARO, Ramat-Yishai 30095, Israel.
Bull Entomol Res. 2007 Aug;97(4):407-13. doi: 10.1017/S0007485307005159.
The sweet potato whitefly, Bemisia tabaci, harbors Portiera aleyrodidarum, an obligatory symbiotic bacterium, as well as several secondary symbionts including Rickettsia, Hamiltonella, Wolbachia, Arsenophonus, Cardinium and Fritschea, the function of which is unknown. Bemisia tabaci is a species complex composed of numerous biotypes, which may differ from each other both genetically and biologically. Only the B and Q biotypes have been reported from Israel. Secondary symbiont infection frequencies of Israeli laboratory and field populations of B. tabaci from various host plants were determined by PCR, in order to test for correlation between bacterial composition to biotype and host plant. Hamiltonella was detected only in populations of the B biotype, while Wolbachia and Arsenophonus were found only in the Q biotype (33% and 87% infection, respectively). Rickettsia was abundant in both biotypes. Cardinium and Fritschea were not found in any of the populations. No differences in secondary symbionts were found among host plants within the B biotype; but within the Q biotype, all whiteflies collected from sage harboured both Rickettsia and Arsenophonus, an infection frequency which was significantly higher than those found in association with all other host plants. The association found between whitefly biotypes and secondary symbionts suggests a possible contribution of these bacteria to host characteristics such as insecticide resistance, host range, virus transmission and speciation.
烟粉虱（Bemisia tabaci）体内携带着一种必需共生细菌——粉虱内共生菌（Portiera aleyrodidarum），以及包括立克次氏体（Rickettsia）、汉密尔顿菌（Hamiltonella）、沃尔巴克氏体（Wolbachia）、阿氏共生菌（Arsenophonus）、卡丁尼亚菌（Cardinium）和弗里茨菌（Fritschea）在内的几种次生共生菌，其功能尚不清楚。烟粉虱是一个由众多生物型组成的复合种，它们在遗传和生物学上可能彼此不同。以色列仅报道了B和Q生物型。通过聚合酶链反应（PCR）测定了来自以色列不同寄主植物的烟粉虱实验室和田间种群的次生共生菌感染频率，以测试细菌组成与生物型和寄主植物之间的相关性。仅在B生物型种群中检测到汉密尔顿菌，而仅在Q生物型中发现了沃尔巴克氏体和阿氏共生菌（感染率分别为33%和87%）。立克次氏体在两种生物型中都很丰富。在任何种群中都未发现卡丁尼亚菌和弗里茨菌。在B生物型的寄主植物之间未发现次生共生菌的差异；但在Q生物型中，从鼠尾草上采集的所有粉虱都同时携带立克次氏体和阿氏共生菌，其感染频率显著高于与所有其他寄主植物相关的粉虱。粉虱生物型与次生共生菌之间的关联表明，这些细菌可能对寄主特性有贡献，如抗药性、寄主范围、病毒传播和物种形成。
