Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2012;7(2):e30760. doi: 10.1371/journal.pone.0030760. Epub 2012 Feb 23.
While every individual of Bemisia tabaci (Hemiptera: Aleyrodidae) harbors the primary symbiont (P-symbiont) Portiera, the infection frequencies of the six secondary symbionts (S-symbionts) including Hamiltonella, Arsenophonus, Cardinium, Wolbachia, Rickettsia and Fritschea vary greatly among different populations. To characterize the factors influencing the infection dynamics of the six S-symbionts in B. tabaci, gene-specific PCR were conducted to screen for the presence of the P-symbiont Portiera and the six S-symbionts in 61 (17 B and 44 Q biotypes) field populations collected from different plant species and locations in China. All individuals of the 61 populations hosted the P-symbiont Portiera, but none of them harbored Arsenophonus and Fritschea. The presence and infection rates of Hamiltonella, Cardinium, Rickettsia, Wolbachia and their co-infections Rickettsia + Hamiltonella (RH), Rickettsia + Cardinium (RC), Hamiltonella + Cardinium (HC) and Rickettsia + Hamiltonella + Cardinium (RHC) varied significantly among the 61 field populations; and the observed variations can be explained by biotypes, sexes, host plants and geographical locations of these field populations. Taken together, at least three factors including biotype, host plant and geographical location affect the infection dynamics of S-symbionts in B. tabaci.
虽然烟粉虱（半翅目：粉虱科）的每个个体都携带主要共生菌（P-共生菌）Portiera，但六种次要共生菌（S-共生菌）包括 Hamiltonella、Arsenophonus、Cardinium、Wolbachia、Rickettsia 和 Fritschea 的感染频率在不同种群中差异很大。为了描述影响 B. tabaci 中六种 S-共生菌感染动态的因素，我们进行了基因特异性 PCR 检测，以筛选 P-共生菌 Portiera 和六种 S-共生菌在 61 个（17 个 B 和 44 个 Q 生物型）从中国不同植物物种和地点采集的田间种群中的存在情况。61 个种群的所有个体都携带 P-共生菌 Portiera，但它们都不携带 Arsenophonus 和 Fritschea。Hamiltonella、Cardinium、Rickettsia、Wolbachia 及其共感染 Rickettsia + Hamiltonella (RH)、Rickettsia + Cardinium (RC)、Hamiltonella + Cardinium (HC) 和 Rickettsia + Hamiltonella + Cardinium (RHC) 的存在和感染率在 61 个田间种群中差异显著；观察到的变异可以用生物型、性别、宿主植物和这些田间种群的地理位置来解释。综上所述，至少有三个因素包括生物型、宿主植物和地理位置影响 B. tabaci 中 S-共生菌的感染动态。
