通过与水溶性碳二亚胺反应对猴病毒40 DNA进行化学修饰。
Chemical modification of simian virus 40 DNA by reaction with a water-soluble carbodiimide.
作者信息
Lebowitz J, Garon C G, Chen M C, Salzman N P
出版信息
J Virol. 1976 Apr;18(1):205-10. doi: 10.1128/JVI.18.1.205-210.1976.
Abstract
Superhelical simian virus 40 (SV40) DNA I can be modified with N-cyclohexyl-N'-beta-(4 methylmorpholinium)ethylcarbodiimide (CMC). The reaction produces an increase in the sedimentation velocity of DNA I from 21 to 22.5S and a decrease in its buoyant density in CsCl from 1.694 to 1.688. A comparable shift in buoyant density is observed in a saturated ethidium bromide-cesium chloride gradient where form II, which has been exposed to CMC, shows no shift. The CsCl-buoyant density data allows us to estimate that 108 mol of CMC are bound per mol of SV40 DNA I. In the subsequent paper an alternative procedure has been used to locate CMC sites, and the extent of the regions available to bind CMC have been measured.
摘要
超螺旋猴病毒40（SV40）DNA I可用N-环己基-N'-β-（4-甲基吗啉鎓）乙基碳二亚胺（CMC）进行修饰。该反应使DNA I的沉降速度从21S增加到22.5S，其在氯化铯中的浮力密度从1.694降低到1.688。在饱和溴化乙锭-氯化铯梯度中观察到类似的浮力密度变化，其中已暴露于CMC的II型没有变化。氯化铯浮力密度数据使我们能够估计每摩尔SV40 DNA I结合108摩尔的CMC。在随后的论文中，已使用另一种方法来定位CMC位点，并测量了可用于结合CMC的区域范围。
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