Van Heuverswyn H, Cole C, Berg P, Fiers W
J Virol. 1979 Jun;30(3):936-41. doi: 10.1128/JVI.30.3.936-941.1979.
Nucleotide sequence analysis of two simian virus 40 early mutants dl1263 and dl1265, which lack a DNA segment around map positions 0.21 and 0.18, respectively (C. Cole, T. Landers, S. Goff, S. MAnteuil-Brutlag, and P. Berg, J. Virol. 24:277--294, 1977), revealed in-phase deletions of 33 nucleotide pairs for dl1263 and 39 nucleotide paris for dl1265. The 33-base-pair deletion in dl1263 does not correspond to an apparent shortening by 6,000 daltons observed for the mutant T antigen. In dl1265 the normal termination signal as well as most of the proline-rich terminal tryptic peptide has been removed, and the carboxyl terminus of the mutant T antigen is a series of three cysteine residues.
对两个猿猴病毒40早期突变体dl1263和dl1265进行的核苷酸序列分析显示,它们分别在图谱位置0.21和0.18附近缺失了一段DNA片段(C. 科尔、T. 兰德斯、S. 戈夫、S. 曼特伊 - 布鲁特拉格和P. 伯格,《病毒学杂志》24:277 - 294,1977),dl1263缺失了33个核苷酸对,dl1265缺失了39个核苷酸对。dl1263中33个碱基对的缺失与突变型T抗原中观察到的明显6000道尔顿的缩短不对应。在dl1265中,正常的终止信号以及大部分富含脯氨酸的末端胰蛋白酶肽都已被去除,突变型T抗原的羧基末端是一系列三个半胱氨酸残基。