Liu Ziyu, Jiang Zhijie, Zhou Kang, Li Pinglan, Liu Guorong, Zhang Bolin
College of Food Science and Nutritional Engineering, China Agricultural University, Room 420, 17# Qinghua East Road, East District, Beijing 100083, China.
Anaerobe. 2007 Oct-Dec;13(5-6):215-9. doi: 10.1016/j.anaerobe.2007.05.002. Epub 2007 May 21.
Tolerance capabilities of 38 Bifidobacterium strains were achieved by simulating the micro-environment of human gastrointestinal tract using modified MRSC broth (pH 3.0). Fourteen strains of them with high viability were obtained in MRSC with 0.3% bile salts. Sequently, six strains of bifidobacteria with higher survivability were picked in MRSC broth (pH 3.0) supplemented with 1-20mg/ml bile salt. Finally, strain A04 with the optimal ability was chosen for further studies. It had been seen that Bifidobacterium breve A04 had better survival capability to 0.5% pepsin (w/v) or 1% pancreatin (w/v) than other bifidobacteria, and viable bacteria were above 8.00 log cfu/ml after incubation for 24h. Meanwhile, it had higher adhesive capability to HT-29 cells in vitro and average adhesive bacteria numbers reached 12.8+/-0.9 for each HT-29 cell. The results indicated that the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells among Bifidobacterium strains was different. B. breve A04 has several aspects of advantages and may be regarded as potential probiotics.
通过使用改良的MRSC肉汤（pH 3.0）模拟人体胃肠道微环境，实现了38株双歧杆菌菌株的耐受能力。其中14株在含有0.3%胆盐的MRSC中具有高活力。随后，在添加了1-20mg/ml胆盐的MRSC肉汤（pH 3.0）中挑选出6株具有更高生存能力的双歧杆菌。最后，选择具有最佳能力的A04菌株进行进一步研究。已观察到，短双歧杆菌A04对0.5%胃蛋白酶（w/v）或1%胰蛋白酶（w/v）的生存能力优于其他双歧杆菌，孵育24小时后活菌数高于8.00 log cfu/ml。同时，它在体外对HT-29细胞具有更高的黏附能力，每个HT-29细胞的平均黏附细菌数达到12.8±0.9。结果表明，双歧杆菌菌株之间耐受胃肠环境的能力和对HT-29细胞的黏附能力存在差异。短双歧杆菌A04具有多个方面的优势，可被视为潜在的益生菌。
