Xie Fei, Gao Bo, Song Cheng-Yi, Chen Guo-Hong
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yi Chuan. 2007 Jul;29(7):785-92. doi: 10.1360/yc-007-0785.
The Sleeping Beauty (SB) transposon is a Tc1/mariner family transposon that has been transpositionally inactive for over 10 million years. The SB transposon system was awakened from inactive Tc1-like transposable elements by using molecular phylogenetic data in 1997. Recent studies on its transposition efficiency and mechanism have shown its broad applications in vertebrate animals for gene-screening, gene transfer, and human gene therapy. In this review, we summarize our current knowledge of Sleeping Beauty, such as structure, transposition mechanism and potential applications, and bring forward some means aiming at the limitations of transposon technology.
“睡美人”（SB）转座子是一种Tc1/水手家族转座子，已经有超过1000万年没有发生转座作用了。1997年，利用分子系统发育数据，从无活性的类Tc1转座元件中唤醒了SB转座子系统。最近关于其转座效率和机制的研究表明，它在脊椎动物的基因筛选、基因转移和人类基因治疗中具有广泛应用。在这篇综述中，我们总结了目前对“睡美人”的认识，如结构、转座机制和潜在应用，并针对转座子技术的局限性提出了一些方法。
