采用反相液相色谱法测定红细胞多胺

Determination of erythrocytic polyamines by reversed-phase liquid chromatography.

作者信息

Gerbaut L

机构信息

Laboratoire de Pharmaco-Biochimie, Hôpital Saint Vincent de Paul, Paris, France.

出版信息

Clin Chem. 1991 Dec;37(12):2117-20.

PMID:1764787

Abstract

A simple and rapid semiautomated procedure for determining polyamines in erythrocytes by high-performance liquid chromatography is described. Putrescine, spermidine, and spermine are converted to fluorescent dansyl derivatives, extracted with cyclohexane, and separated in less than 10 min on a reversed-phase C18 ODS column, with an acetonitrile-water gradient as the mobile phase. The method showed a coefficient of variation of 2.73% for spermidine and 3.27% for spermine. The respective reference values, evaluated in 10 healthy patients, were 7.88 (SD 2.09) and 5.42 (SD 1.55) mumol/L of packed erythrocytes. Only negligible amounts of putrescine were found.

摘要

本文描述了一种通过高效液相色谱法测定红细胞中多胺的简单快速的半自动方法。腐胺、亚精胺和精胺被转化为荧光丹磺酰衍生物，用环己烷萃取，并在反相C18 ODS柱上以乙腈-水梯度作为流动相在不到10分钟内分离。该方法显示亚精胺的变异系数为2.73%，精胺的变异系数为3.27%。在10名健康患者中评估的各自参考值分别为每升压积红细胞7.88（标准差2.09）和5.42（标准差1.55）微摩尔。仅发现极少量的腐胺。