Kai M, Ogata T, Haraguchi K, Ohkura Y
J Chromatogr. 1979 Jun 11;163(2):151-60. doi: 10.1016/s0378-4347(00)81458-9.
A highly sensitive and simple fluorimetric method for the determination of free and total polyamines, spermidine, spermine, putrescine and cadaverine, in human serum by high-performance liquid chromatography is described. The polyamines, obtained after clean-up of deproteinized serum by Cellex P column chromatography, are converted to their fluorescamine derivatives in the presence of nickel ion which inhibits the reaction of interfering amines with fluorescamine, and the derivatives are separated simultaneously by reversed-phase chromatography (LiChrosorb RP-18) with a linear gradient elution. The lower limits of detection are 10 and 15 pmole for spermine and the others in 0.5 ml of serum, respectively.
描述了一种通过高效液相色谱法测定人血清中游离和总多胺（亚精胺、精胺、腐胺和尸胺）的高灵敏度且简单的荧光法。通过Cellex P柱色谱法对脱蛋白血清进行净化后得到的多胺，在镍离子存在下转化为它们的荧光胺衍生物，镍离子可抑制干扰胺与荧光胺的反应，这些衍生物通过反相色谱法（LiChrosorb RP - 18）采用线性梯度洗脱同时进行分离。在0.5 ml血清中，精胺及其他多胺的检测下限分别为10和15皮摩尔。
