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一种改良荧光抗体法在动物组织中钩端螺旋体检测中的应用

The Use of an Improved Fluorescent Antibody Procedure in the Demonstration of Leptospira in Animal Tissues.

作者信息

Maestrone G

出版信息

Can J Comp Med Vet Sci. 1963 May;27(5):108-12.

Abstract

Increased sensitivity of the fluorescent antibody procedure was achieved by modification of the method employed. Specimens collected from experimentally infected laboratory animals and naturally infected domestic and wild animals were examined by means of this method. The results compared very favorably with those obtained by other investigators who used serological, cultural, animal inoculation and silver impregnation procedures on the same specimens.

摘要

通过对所用方法进行改进,提高了荧光抗体检测程序的灵敏度。采用该方法对从实验感染的实验动物以及自然感染的家畜和野生动物采集的标本进行了检测。与其他研究人员在相同标本上使用血清学、培养、动物接种和银浸染程序所获得的结果相比,本研究结果非常有利。

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本文引用的文献

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Detection of Leptospira pomona in guinea pig and bovine urine with fluorescein-labeled antibody.
J Infect Dis. 1959 Sep-Oct;105:118-23. doi: 10.1093/infdis/105.2.118.
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Use of contrasting fluorescent dye as counterstain in fixed tissue preparations.
Proc Soc Exp Biol Med. 1959 Oct;102:179-81. doi: 10.3181/00379727-102-25182.
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Fluorescent treponemal antibody test. Modification based on quantitation (FTA-200).
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