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用于检测自然感染绵羊中弓形虫感染的各种血清学检测方法的敏感性和特异性。

Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep.

作者信息

Shaapan R M, El-Nawawi F A, Tawfik M A A

机构信息

Department of Zoonosis, National Research Center, Giza, Egypt.

出版信息

Vet Parasitol. 2008 May 31;153(3-4):359-62. doi: 10.1016/j.vetpar.2008.02.016. Epub 2008 Mar 4.

DOI:10.1016/j.vetpar.2008.02.016
PMID:18406534
Abstract

Comparative serological examination of 300 serum samples from sheep slaughtered in the main abattoir in Cairo, Egypt revealed a higher prevalence of toxoplasmosis (43.7%) with the modified agglutination test (MAT), followed by the enzyme linked immune-sorbant assay (ELISA) (41.7%) and the indirect fluorescent antibody test (IFAT) (37%), while the lowest prevalence was detected with the dye test (DT) (34%). When the data from the first three serological tests were compared with that of the DT test, which was used as a reference test for toxoplasmosis, MAT had the highest sensitivity (96%), followed by ELISA (90.1%) and IFAT, which demonstrated the lowest sensitivity (80.4%). Conversely, IFAT had the highest specificity (91.4%), followed by MAT (88.9%) and ELISA (85.9%).

摘要

对来自埃及开罗主要屠宰场宰杀的300份绵羊血清样本进行的比较血清学检查显示,改良凝集试验(MAT)检测到的弓形虫病患病率较高(43.7%),其次是酶联免疫吸附测定(ELISA)(41.7%)和间接荧光抗体试验(IFAT)(37%),而染料试验(DT)检测到的患病率最低(34%)。当将前三项血清学检测的数据与用作弓形虫病参考检测的DT检测数据进行比较时,MAT的灵敏度最高(96%),其次是ELISA(90.1%),IFAT的灵敏度最低(80.4%)。相反,IFAT的特异性最高(91.4%),其次是MAT(88.9%)和ELISA(85.9%)。

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