Ding Jing-juan, Liu Yue-hui, Wang Mei
Department of Infectious Diseases, Guiyang Medical College Affiliated Hospital, Guiyang 550004, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 Feb;28(2):169-72.
To investigate the distribution of hepatitis B virus (HBV) precore A1896 and basic core promoter (BCP)T1762/A1764 mutations in Guizhou area.
482 patients with chronic HBV infection, belonging to 4 nationalities, including 225 asymptomatic carriers (ASC), 158 chronic hepatitis (CH), 57 liver cirrhosis (LC), 42 hepatocellular carcinoma (HCC), from 4 areas of Guizhou province were examined. HBV A1896 and T1762/A1764 mutations were determined by direct sequencing and restriction fragment length polymorphism (RFLP). HBV genotypes were determined by PCR-RFLP based on S gene. The relationship among these mutations and genotype and the progression of liver disease were studied by multi-normal logistic regression analysis.
A1896 and T1762/A1764 mutations were detected 23.03% and 29.67% among 482 patients. These mutations were more prevalent in Hans than in Dong, Miao and Buyi minorities (P < 0.01, respectively). The mutations of A1896 and T1762/ A1764 were more commonly seen in HBeAg negative than in HBeAg positive patients (P < 0.01, respectively). The mutation of T1762/A1764 was significantly higher in genotype C than in genotype B (P < 0.01). There were significantly statistical differences in the detective rate of A1896 and T1762/ A1764 mutations between patients with HCC, LC and CH, ASC. The distribution of these mutations in Guiyang (31.79% and 41.06%) was higher than in Zunye (10.94%, 14.06%), Duyun (8.64%, 11.11%) or Kaili (2.86%, 2.86%). However, there was no statistical difference by multi-normal logistic regression analysis after controlling the influence of HBeAg statu, genotype and clinical types.
The distributions of A1896 and T1762/A1764 mutations were different in some nationalities of Guizhou province. The mutation of T1762/A1764 was more commonly seen in genotype C than in genotypr B. These mutations were closely related to progression of chronic liver diseases. Hepatitis B virus; Genotype; Restriction fragment length polymorphism
探讨贵州地区乙型肝炎病毒（HBV）前核心A1896和基本核心启动子（BCP）T1762/A1764突变的分布情况。
对来自贵州省4个地区的482例慢性HBV感染者进行检测，这些患者分属4个民族，包括225例无症状携带者（ASC）、158例慢性肝炎（CH）、57例肝硬化（LC）、42例肝细胞癌（HCC）。采用直接测序和限制性片段长度多态性（RFLP）法检测HBV A1896和T1762/A1764突变。基于S基因采用PCR-RFLP法确定HBV基因型。通过多因素logistic回归分析研究这些突变与基因型及肝病进展之间的关系。
在482例患者中，A1896和T1762/A1764突变的检出率分别为23.03%和29.67%。这些突变在汉族中比在侗族、苗族和布依族中更常见（P均<0.01）。A1896和T1762/A1764突变在HBeAg阴性患者中比在HBeAg阳性患者中更常见（P均<0.01）。T1762/A1764突变在C基因型中显著高于B基因型（P<0.01）。HCC、LC、CH和ASC患者中A1896和T1762/A1764突变的检出率存在显著统计学差异。这些突变在贵阳的分布（31.79%和41.06%）高于遵义（10.94%，14.06%）、都匀（8.64%，11.11%）或凯里（2.86%，2.86%）。然而，在控制HBeAg状态、基因型和临床类型的影响后，多因素logistic回归分析无统计学差异。
贵州部分民族中A并1896和T1762/A1764突变的分布不同。T1762/A1764突变在C基因型中比在B基因型中更常见。这些突变与慢性肝病的进展密切相关。乙型肝炎病毒；基因型；限制性片段长度多态性
