Zhou Bin, Wang Zhan-hui, Chen Jin-jun, Huang Yue-hua, Sun Jian, Hou Jin-lin
Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Zhonghua Gan Zang Bing Za Zhi. 2008 Mar;16(3):203-6.
To investigate the clinical characteristics and the pattern of precore and core promoter mutations of hepatitis B virus (HBV) subgenotypes Ba, C1 and C2.
A cohort of 151 patients with chronic HBV infection in Guangdong province of China was enrolled in this study. HBV subgenotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Precore and core promoter mutations were analysed using nucleotide sequencing.
Of the 151 patients, 80, 51 and 20 were infected with subgenotypes Ba, C1 and C2 respectively. No significant differences were found in HBeAg positivity and liver functional indexes among these three subgenotypes when age and sex were matched. Virologically, HBV/Ba showed the highest frequency of A1896 mutation but the lowest frequency of T1762/A1764 mutation. HBV/C1 was associated with the highest tendency to develop T1762/A1764 mutation, but the lowest prevalence of A1896 mutation. HBV/C2 was associated with an intermediate tendency to develop A1896 and T1762/A1764 mutations.
Different mutation patterns in precore and core promoter regions are responsible for HBeAg-negative HBV infections among different subgenotypes.
研究乙型肝炎病毒（HBV）Ba、C1和C2亚基因型的临床特征以及前核心和核心启动子突变模式。
本研究纳入了中国广东省151例慢性HBV感染患者。通过聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）确定HBV亚基因型。使用核苷酸测序分析前核心和核心启动子突变。
151例患者中，分别有80例、51例和20例感染Ba、C1和C2亚基因型。在年龄和性别匹配的情况下，这三种亚基因型的HBeAg阳性率和肝功能指标未发现显著差异。在病毒学方面，HBV/Ba的A1896突变频率最高，但T1762/A1764突变频率最低。HBV/C1发生T1762/A1764突变的倾向最高，但A1896突变的患病率最低。HBV/C2发生A1896和T1762/A1764突变的倾向处于中等水平。
前核心和核心启动子区域的不同突变模式导致了不同亚基因型中HBeAg阴性的HBV感染。
