特发性血小板减少性紫癜患者CD4+ CD25+调节性T细胞的改变

[Alterations of CD4+ CD25+ regulatory T cells in patients with idiopathic thrombocytopenic purpura].

作者信息

Ling Yun, Cao Xiang-Shan, Yu Zi-Qiang, Luo Guang-Hua, Bai Xia, Su Jian, Dai Lan, Ruan Chang-Geng

机构信息

The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology , Suzhou 215006, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2007 Mar;28(3):184-8.

PMID:17649712

Abstract

OBJECTIVE

To investigate the role of CD4+ CD25+ regulatory T (Treg) cells in patients with ITP.

METHODS

Flow cytometry was used to examine the number of CD4+ CD25+, CD4+ CD25 high, CD4+ Foxp3+ and CD4+ CD25+ Foxp3+ T cells. The level of Foxp3 mRNA expression was analyzed by realtime quantitative reverse transcriptase polymerase chain reaction (RT-PCR) . CD4+ CD25 high T cells was cocultured with CD4+ CD25 - T cells from patients or controls for assessing the regulatory properties of CD4+ CD25 Treg cells.

RESULTS

The proportion of CD4+ CD25+ T cells in the peripheral blood of patients with ITP [(15.64 +/- 5.82) %] was significantly higher than that in normal control group [(9.30 +/- 3.95)%] (P <0.01). There was no significant difference in the percentages of CD4+ CD25 high T cells between ITP patients and controls [(1.53 +/- 0.66)% versus (1.36 +/- 0.55)% (P = 0.317)]. But the number of CD4 Foxp 3+ T cells and CD4 + CD25+ Foxp3+ T cells in patients were significantly lower than that in control group (P <0.01). The expression of Foxp3 mRNA reduced (P < 0.01) and the suppressive activity of CD4+ CD25 high T cells is lower than that of healthy controls (P <0.01).

CONCLUSION

In patients with ITP, both the number and immuno-regulative function of CD4+ CD25+ Treg cells are reduced.

摘要

目的

探讨CD4+CD25+调节性T（Treg）细胞在免疫性血小板减少症（ITP）患者中的作用。

方法

采用流式细胞术检测CD4+CD25+、CD4+CD25高表达、CD4+Foxp3+及CD4+CD25+Foxp3+T细胞数量。通过实时定量逆转录聚合酶链反应（RT-PCR）分析Foxp3 mRNA表达水平。将CD4+CD25高表达T细胞与患者或对照组的CD4+CD25-T细胞共培养，以评估CD4+CD25 Treg细胞的调节特性。

结果

ITP患者外周血中CD4+CD25+T细胞比例[（15.64±5.82）%]显著高于正常对照组[（9.30±3.95）%]（P<0.01）。ITP患者与对照组之间CD4+CD25高表达T细胞百分比无显著差异[（1.53±0.66）%对（1.36±0.55）%（P = 0.317）]。但患者的CD4 Foxp3+T细胞和CD4+CD25+Foxp3+T细胞数量显著低于对照组（P<0.01）。Foxp3 mRNA表达降低（P<0.01），CD4+CD25高表达T细胞的抑制活性低于健康对照组（P<0.01）。