Paranhos H F O, Silva-Lovato C H, Souza R F, Cruz P C, Freitas K M, Peracini A
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Oral Rehabil. 2007 Aug;34(8):606-12. doi: 10.1111/j.1365-2842.2007.01753.x.
The aim of this study was to quantify biofilm on the internal surface of upper complete dentures following six possible cleansing methods. Thirty-six edentulous subjects were submitted to a time-series trial and dentures were cleansed according to six methods: (i) rinsing with water; (ii) soaking in an alkaline peroxide solution (Bonyplus); (iii) brushing with dentifrice (Dentu-Creme) and soft Johnson and Johnson's toothbrush; (iv) combination between soaking and brushing according to methods 2 and 3; (v) brushing with dentifrice (Dentu-Creme) and soft Oral B toothbrush; (vi) combination between soaking and brushing according to methods 2 and 5. Each method was randomly used throughout 21 days. Denture biofilm was disclosed by 1% neutral red solution and quantified by means of digital photos taken from the internal surface. The six methods presented significant differences in percentage of biofilm coverage (repeated measures anova, P < 0.0001). Method 1 showed the highest values, 2 was intermediate and other results were the lowest. The most efficacious approach was 6. Biofilm tended to accumulate predominantly over specific zones of the denture base, but this pattern did not change regardless of the method employed. It can be concluded that brushing alone was more effective than the chemical method employed. The best results were obtained by a combination of methods.
本研究的目的是对采用六种可能的清洁方法后的上颌全口义齿内表面的生物膜进行量化。36名无牙颌受试者参与了一项时间序列试验，义齿根据六种方法进行清洁：（i）用水冲洗；（ii）浸泡在碱性过氧化物溶液（Bonyplus）中；（iii）用牙膏（Dentu-Creme）和强生软质牙刷刷牙；（iv）按照方法2和3进行浸泡和刷牙相结合；（v）用牙膏（Dentu-Creme）和欧乐B软质牙刷刷牙；（vi）按照方法2和5进行浸泡和刷牙相结合。在21天内随机使用每种方法。用1%中性红溶液显示义齿生物膜，并通过从内表面拍摄的数码照片进行量化。六种方法在生物膜覆盖百分比方面存在显著差异（重复测量方差分析，P < 0.0001）。方法1显示的值最高，方法2居中，其他结果最低。最有效的方法是方法6。生物膜倾向于主要积聚在义齿基托的特定区域，但无论采用何种方法，这种模式都没有改变。可以得出结论，单独刷牙比采用的化学方法更有效。通过方法组合可获得最佳结果。
