Paranhos Helena F Oliveira, Silva-Lovato Cláudia H, de Souza Raphael F, Cruz Patricia C, de Freitas-Pontes Karina M, Watanabe Evandro, Ito Izabel Y
Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, University of São Paulo, Brazil.
J Prosthodont. 2009 Jul;18(5):427-31. doi: 10.1111/j.1532-849X.2009.00450.x. Epub 2009 Mar 26.
The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens.
The set (sterile stainless steel basket and specimens) was contaminated (37 degrees C for 48 hours) by a microbial inoculum with 10(6) colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 degrees C for 48 hours.
Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results.
The three denture hygiene methods showed different effects depending on the type of microbial biofilms formed on acrylic base resin specimens.
本研究旨在评估三种义齿清洁方法对在丙烯酸树脂标本上形成的不同微生物生物膜的效果。
将一组（无菌不锈钢篮和标本）用含有10(6)个菌落形成单位（CFU）/毫升的微生物接种物污染（37℃，48小时）（标准菌株：金黄色葡萄球菌、变形链球菌、大肠杆菌、白色念珠菌、铜绿假单胞菌和粪肠球菌；现场菌株：变形链球菌、白色念珠菌、光滑念珠菌和热带念珠菌）。接种后，标本通过以下方法进行清洁：（1）化学方法：浸入碱性过氧化物溶液（Bonyplus片）中5分钟；（2）机械方法：用用于可摘义齿的牙膏（Dentu Creme）刷牙20秒；（3）化学和机械方法相结合。将标本置于含有适当培养基的培养皿上10分钟。之后，取出标本，将培养皿在37℃下孵育48小时。
化学、机械和联合方法在减少金黄色葡萄球菌、变形链球菌（ATCC和现场菌株）和铜绿假单胞菌的CFU方面没有显著差异。机械和联合方法相似，并且在减少粪肠球菌、白色念珠菌（ATCC和现场菌株）和光滑念珠菌方面比化学方法更有效。联合方法在减少大肠杆菌和热带念珠菌方面比化学方法更好，机械方法显示出中等效果。
三种义齿清洁方法根据在丙烯酸基树脂标本上形成的微生物生物膜类型显示出不同的效果。
