Wu Sheng-Long, Bao Wen-Bin, Ju Hui-Ping, Chen Guo-Hong, Li Bi-Chun, Cheng Jin-Hua, Zhu Guo-Qiang
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yi Chuan. 2007 Jun;29(6):693-8. doi: 10.1360/yc-007-0693.
By the PCR-RFLP method, the polymorphisms of exon 14 of Mx1 gene were detected in 7 native and foreign pig breeds. Taken together with the analysis of restriction enzyme Hin6 I digestion, there were 6 genotypes and 3 alleles. And, all individuals of Duroc exhibited the AA genotype but the Sutai pigs exhibited all three kinds of genotypes. The BB genotype was detected only in Meishan pigs and their derivate Sutai pigs. Among all pig breeds, the allele B only appeared in Chinese indigenous pig breeds and developed pig breed in this study, and the allele A was dominant allele in all pig breeds except for Songliao black pig. The results of Chi Square test showed that there were abundant polymorphisms in all pig breeds. The gene frequency of Meishan pig and Songliao black pig showed greatly significant difference to other pig breeds (P<0.01), Sutai pig showed greatly significant difference (P<0.01) to other pig breeds except for Pietrain; and Huai pig showed greatly significant differences (P<0.01) with Pietrain and other indigenous Chinese pig breeds, but no difference with Duroc and Yorkshire (P>0.05). Three new mutation points in three genotypes were identified by sequencing comparison analysis of PCR products, two of which resulted in Thr-->Ala and Glu-->Arg respectively, the last one was a silence mutation, the Glu-->Arg mutation point and this silence mutation point only appeared in the individuals with the BB genotype.
采用PCR-RFLP方法，对7个国内外猪种Mx1基因外显子14的多态性进行了检测。结合限制性内切酶Hin6Ⅰ酶切分析，共检测到6种基因型和3个等位基因。杜洛克猪所有个体均表现为AA基因型，而苏太猪表现出三种基因型。BB基因型仅在梅山猪及其培育品种苏太猪中被检测到。在本研究的所有猪种中，等位基因B仅出现在中国地方猪种和培育猪种中，除了松辽黑猪外，等位基因A在所有猪种中均为优势等位基因。卡方检验结果表明，所有猪种均存在丰富的多态性。梅山猪和松辽黑猪的基因频率与其他猪种差异极显著（P<0.01），苏太猪与除皮特兰猪外的其他猪种差异极显著（P<0.01）；淮猪与皮特兰猪及其他中国地方猪种差异极显著（P<0.01），但与杜洛克猪和大白猪无差异（P>0.05）。通过对PCR产物进行测序比较分析，在三种基因型中鉴定出三个新的突变位点，其中两个分别导致Thr→Ala和Glu→Arg，最后一个是沉默突变，Glu→Arg突变位点和这个沉默突变位点仅出现在BB基因型个体中。
