Pan Zhang-Yuan, Ye Lan, Zhu Jing, DU Zi-Dong, Huang Xiao-Guo, Zhu Guo-Qian, Bao Wen-Bin, Wu Sheng-Long
Animal Science and Technology College, Yangzhou University, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Besign of Jiangsu Province, Yangzhou 225009, China.
Yi Chuan. 2011 Feb;33(2):163-7. doi: 10.3724/sp.j.1005.2011.00163.
Using the PCR-SSCP method, the genetic variation in exon 1 of the TLR4 gene was detected among 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous swine breeds. This was conducted to analyze the polymorphisms of exon 1 of TLR4 gene in native and foreign pig breeds and aimed at providing a theoretical foundation for further research on the role that TLR4 gene played in immune and defense system. New alleles were isolated for exon 1 of the swine TLR4 gene for the first time, There were 6 genotypes and 3 alleles, in which the Duroc appeared AA, BB, CC, AB, AC and BC genotypes; Sutai pig, which has Duroc pig origin, were detected to be BB, CC, and BC genotypes; Yorkshire and Landrace were detected to be CC and BC genotypes. Wild boar and all 10 Chinese native pig breeds appeared highly conserved in exon 1 of TLR4 gene, with only CC genotype. Among the 3 homozygous genotypes, the CC genotype matches the sequence in GenBank, while a G93C synonymous mutation and a G194A nonsense mutation were found in the BB and AA genotypes, respectively. The correlation between these two mutation points of TLR4 gene with resistance to stress and disease is worthy of further study.
采用聚合酶链反应-单链构象多态性（PCR-SSCP）方法，在893头动物中检测了TLR4基因外显子1的遗传变异，这些动物包括亚洲野猪、3个引进的商业猪种和10个中国本土猪种。旨在分析国内外猪种TLR4基因外显子1的多态性，为进一步研究TLR4基因在免疫和防御系统中的作用提供理论基础。首次分离出猪TLR4基因外显子1的新等位基因，有6种基因型和3个等位基因，其中杜洛克猪出现AA、BB、CC、AB、AC和BC基因型；有杜洛克猪血统的苏太猪检测到为BB、CC和BC基因型；大白猪和长白猪检测到为CC和BC基因型。野猪和所有10个中国本土猪种在TLR4基因外显子1中表现出高度保守，仅出现CC基因型。在3种纯合基因型中，CC基因型与GenBank中的序列匹配，而在BB和AA基因型中分别发现了一个G93C同义突变和一个G194A无义突变。TLR4基因这两个突变点与应激和疾病抗性之间的相关性值得进一步研究。
