Zhu Xiao-Dong, Geng Bo, Li Jiao, Sun Xiao-Wen
Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Science, Harbin 150070, China.
Yi Chuan. 2007 Jun;29(6):705-13. doi: 10.1360/yc-007-0705.
Thirty microsatellite markers were used to analyze the genetic diversity of five Silver carp populations in the middle and lower reaches of the Yangtze River. A total of 144 different alleles were found and the number of alleles in each locus ranged from 1 to 10. Twenty-five loci (83.33%) were polymorphic. In the five populations, the average number of alleles was 4.0 to 4.1, the number of mean valid alleles was 2.4445 to 2.6332, the value of average observed and expected heterozygosity ranged from 0.3233 to 0.3511 and 0.4421 to 0.4704, and the mean PIC was 0.4068 to 0.4286. Fst value indicated that the populations were moderately differentiated. Chi-square test was used to analyze the genotypes based on Hardy-Weinberg equilibrium, the P value denoted that the five populations deviated equilibrium partially. The genetic similarity coefficient of the five populations was 0.8466 to 0.9146, and the genetic distance of the populations was 0.0893 to 0.1665. A UPGMA dendrogram was constructed based on Nei's standard genetic distance for the five Silver carp populations.
利用30个微卫星标记对长江中下游5个鲢群体的遗传多样性进行了分析。共检测到144个不同等位基因，每个位点的等位基因数为1至10个。25个位点（83.33%）具有多态性。在这5个群体中，平均等位基因数为4.0至4.1，平均有效等位基因数为2.4445至2.6332，平均观测杂合度和期望杂合度值分别为0.3233至0.3511和0.4421至0.4704，平均多态信息含量为0.4068至0.4286。Fst值表明群体间存在中等程度的分化。采用卡方检验分析基于哈迪-温伯格平衡的基因型，P值表明这5个群体部分偏离平衡。5个群体的遗传相似系数为0.8466至0.9146，群体间的遗传距离为0.0893至0.1665。基于Nei氏标准遗传距离构建了5个鲢群体的UPGMA聚类图。
