[三氧化二砷对K562细胞中VEGF/R及MMP-2、9表达的影响]

[Effect of arsenic trioxide on VEGF/R and MMP-2, 9 expressed in K562 cells].

作者信息

Zhang Yu, Zhang Jian-Dong, Gu Jian, Ma Li, Shen Wei-Gan, Wang Xiao-Ling, Chen Hong

机构信息

Clinic Medical College of Yangzhou University, Yangzhou Institute of Hematology, Yangzhou 225001, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2007 Feb;28(2):107-10.

PMID:17650671

Abstract

OBJECTIVE

To explore the effect of arsenic trioxide (As2 O3) on the level of VEGF, VEGFR and the activity of MMP-2, 9 in K562 cells.

METHODS

The inhibition ratio of K562 cell was detected by MTT assay, the level of VEGF by Enzyme-linked immunosorbent assay (ELISA), the expression ratio of VEGFR by flow cytometry (FCM), and the activity of MMP-2, 9 by gelatin zymography assay.

RESULTS

(1) The IC50 of K562 cells was (2.12 +/- 0.11) micromol/L. Proliferation of K562 cells was significantly inhibited at the concentration of 0.4 - 6.4 micromol/L As2 O3 (P < 0.05). (2) The expression of VEGF was slightly up-regulated by 0.05 micromol/L As2 O3 (P > 0.05) and prominently inhibited by 0.4 micromol/L and 3.2 micromol/L As2 O3 (P < 0.05). As2 O3 had no influence on VEGFR. (3) The activity of MMP-2 and 9 was partly inhibited by 0.05 micromol/L As2 O3 incubated 72 hours and by 0.4, 3.2 micromol/L, As2 O3. With the increase of As2 O3 concentration and the incubation time, the inhibited effect on MMP-2 and 9 was enhanced.

CONCLUSIONS

As2 O3 may down-regulate the expression of VEGF and inhibit the activity of MMP-2 and 9.

摘要

目的

探讨三氧化二砷（As2O3）对K562细胞中血管内皮生长因子（VEGF）、血管内皮生长因子受体（VEGFR）水平及基质金属蛋白酶-2、9（MMP-2、9）活性的影响。

方法

采用MTT法检测K562细胞的抑制率，酶联免疫吸附测定（ELISA）法检测VEGF水平，流式细胞术（FCM）检测VEGFR的表达率，明胶酶谱法检测MMP-2、9的活性。

结果

（1）K562细胞的半数抑制浓度（IC50）为（2.12±0.11）μmol/L。0.4～6.4μmol/L As2O3浓度可显著抑制K562细胞增殖（P<0.05）。（2）0.05μmol/L As2O3可使VEGF表达轻度上调（P>0.05），0.4μmol/L和3.2μmol/L As2O3可显著抑制VEGF表达（P<0.05）。As2O3对VEGFR无影响。（3）0.05μmol/L As2O3作用72小时及0.4、3.2μmol/L As2O3可部分抑制MMP-2、9的活性。随着As2O3浓度增加及作用时间延长，对MMP-2、9的抑制作用增强。