Liang Hong, Zhang Yu, Zhang Jian-dong, Gu Jian, Ma Li, Wang Xiao-ling, Shen Wei-gan
Medical College, Yangzhou University, Yangzhou, Jiangsu Province 225001, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Nov;5(6):647-50. doi: 10.3736/jcim20070609.
To investigate the effects of arsenic trioxide (ATO) on the expressions of vascular endothelial growth factor (VEGF) and P-glycoprotein (P-gp) in K562/A02 cells and to explore the correlation between VEGF and P-gp.
The inhibition rate of K562/A02 cell proliferation was detected by using methyl thiazolyl tetrazolium assay (MTT); the level of VEGF was detected by enzyme-linked immunosorbent assay (ELISA) and the expression rate of P-gp was determined by flow cytometry (FCM).
0.05 micromol/L ATO had no influences on the cell proliferation and the expression of VEGF in K562/A02 cells; 0.4 and 3.2 micromol/L ATO could significantly inhibit the K562/A02 cell proliferation and down-regulate the expression of VEGF in K562/A02 cells (P<0.05). The expression of P-gp did not changed after being exposed to 0.05 and 0.4 micromol/L ATO for 24, 48 and 72 hours (P>0.05). 3.2 micromol/L ATO could remarkably reduce the expression of P-gp in K562/A02 cells after 48- and 72-hour incubation with ATO (P<0.05).
The down-regulation of P-gp and VEGF after being exposed to ATO probably contributes to the reversion of multidrug resistance in K562/A02 cells. The level of VEGF may be related to the expression rate of P-gp in K562/A02 cells.
研究三氧化二砷（ATO）对K562/A02细胞中血管内皮生长因子（VEGF）和P-糖蛋白（P-gp）表达的影响，并探讨VEGF与P-gp之间的相关性。
采用甲基噻唑基四氮唑蓝比色法（MTT）检测K562/A02细胞增殖抑制率；采用酶联免疫吸附测定法（ELISA）检测VEGF水平，采用流式细胞术（FCM）测定P-gp表达率。
0.05 μmol/L ATO对K562/A02细胞增殖及VEGF表达无影响；0.4和3.2 μmol/L ATO可显著抑制K562/A02细胞增殖，并下调K562/A02细胞中VEGF的表达（P<0.05）。在0.05和0.4 μmol/L ATO作用24、48和72小时后，P-gp的表达未发生变化（P>0.05）。在与3.2 μmol/L ATO孵育48和72小时后，K562/A02细胞中P-gp的表达显著降低（P<0.05）。
ATO作用后P-gp和VEGF的下调可能有助于K562/A02细胞多药耐药性的逆转。VEGF水平可能与K562/A02细胞中P-gp的表达率有关。
