Ali B, Hasnain S
Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.
Lett Appl Microbiol. 2007 Aug;45(2):128-33. doi: 10.1111/j.1472-765X.2007.02158.x.
The main aim of this study was to investigate the possible role of indoleacetic acid (IAA) from bacteria to induce in vitro adventitious shoots in internodal explants of Brassica oleracea L.
Culture supernatant of Halomonas sp. RE1 and Halomonas sp. HT1 that contain 21 and 40 microg ml(-1) IAA, respectively, was used to supplement Murashige and Skoog (MS) medium. Two combinations that were supplemented with bacterial supernatant (BS) are MS + BS and MS + BS + 10%CW (coconut water) while basal MS medium was used as control. The amounts of BS used in this experiment were 50, 100, 150 and 200 microl in 5 ml MS medium in each combination. In vitro-grown internodal explants of B. oleracea were inoculated on these media combinations and incubated in a growth chamber at 25 +/- 1 degrees C and exposed to 16-h cool fluorescent light. After 5-6 weeks of incubation adventitious shoot induction was observed in all treatments that were supplemented with BS as compared with the controls where very low response was observed. The frequency of shoot induction was high in media that were supplemented with 10%CW in the presence of bacterial auxin.
It was concluded that IAA of microbial origin has the potential to induce adventitious shoots in internodal explants.
IAA from bacteria can be effectively used in plant tissue culture; especially a combination of MS + BS + 10%CW is very cost-effective as compared with synthetic phytohormones for in vitro studies.
本研究的主要目的是探究细菌产生的吲哚乙酸（IAA）在甘蓝型油菜节间外植体中诱导不定芽的潜在作用。
分别含有21和40微克/毫升IAA的盐单胞菌属RE1和盐单胞菌属HT1的培养上清液用于补充Murashige和Skoog（MS）培养基。两种添加细菌上清液（BS）的组合为MS + BS和MS + BS + 10%椰汁（CW），而基础MS培养基用作对照。本实验中，每种组合在5毫升MS培养基中使用的BS量分别为50、100、150和200微升。将甘蓝型油菜的离体节间外植体接种在这些培养基组合上，并在25±1℃的生长室中培养，置于16小时冷荧光灯下。培养5 - 6周后，与对照相比，在所有添加BS的处理中均观察到不定芽诱导，对照的响应非常低。在添加细菌生长素的情况下，添加10%椰汁的培养基中芽诱导频率较高。
得出微生物来源的IAA有潜力在节间外植体中诱导不定芽的结论。
细菌来源的IAA可有效用于植物组织培养；特别是与用于离体研究的合成植物激素相比，MS + BS + 10%椰汁的组合非常具有成本效益。
