Chen Y-S, Srionnual S, Onda T, Yanagida F
Institute of Enology and Viticulture, University of Yamanashi, Yamanashi, Japan.
Lett Appl Microbiol. 2007 Aug;45(2):190-3. doi: 10.1111/j.1472-765X.2007.02167.x.
To investigate the effects of two prebiotics and trehalose on the production of bacteriocins.
Four carbohydrates [dextrose, fructo-oligosaccharides (FOS), raffinose, and trehalose] were used as the sole carbon source in a simple broth. Five bacteriocin-producing strains of bacteria, including those producing nisin, enteriocin, and other bacteriocins, were used, and their inhibitory activities when grown on each carbohydrate were determined. The inhibitory activity assay was performed using the agar well diffusion method, and Lactobacillus sakei JCM 1,157(T) was used as the indicator strain. Effective enhancement of bacteriocin production was observed with FOS and trehalose incubation.
The results suggest that FOS and trehalose can effectively enhance the production of the five kinds of bacteriocins evaluated in this study.
This study offers useful information for not only a new application of FOS and trehalose, but also the potential improvement of food preservation.
研究两种益生元和海藻糖对细菌素产生的影响。
在一种简单肉汤中使用四种碳水化合物[葡萄糖、低聚果糖（FOS）、棉子糖和海藻糖]作为唯一碳源。使用了五株产生细菌素的菌株，包括那些产生乳酸链球菌素、肠菌素和其他细菌素的菌株，并测定了它们在每种碳水化合物上生长时的抑制活性。采用琼脂孔扩散法进行抑制活性测定，并使用清酒乳杆菌JCM 1157(T)作为指示菌株。观察到用FOS和海藻糖培养可有效提高细菌素的产量。
结果表明，FOS和海藻糖可有效提高本研究中评估的五种细菌素的产量。
本研究不仅为FOS和海藻糖的新应用，也为食品保鲜的潜在改善提供了有用信息。
