Norry F M, Sambucetti P, Scannapieco A C, Gomez F H, Loeschcke V
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Insect Mol Biol. 2007 Aug;16(4):509-13. doi: 10.1111/j.1365-2583.2007.00747.x.
Knockdown Resistance to High Temperature (KRHT) is an adaptive trait of thermotolerance in insects. An interval mapping was performed on chromosome X of Drosophila melanogaster to search for quantitative trait loci (QTL) affecting KRHT. A backcross population was obtained from two lines that dramatically differ for KRHT. Microsatellites were used as markers. Composite interval mapping identified a large-effect QTL in the region of band 10 where putative candidate genes map. To further test for this QTL a set of recombinant (but non-inbred) lines was obtained from backcrosses between the parental lines used for the interval mapping. Recombinant line analysis confirmed that one QTL is targeted by band 10. We identify and discuss candidate loci contained within our QTL region.
抗高温击倒（KRHT）是昆虫耐热性的一种适应性特征。对黑腹果蝇的X染色体进行区间作图，以寻找影响KRHT的数量性状基因座（QTL）。从两个KRHT差异极大的品系获得了一个回交群体。微卫星被用作标记。复合区间作图在10区带区域鉴定出一个具有显著效应的QTL，该区域有假定的候选基因定位。为了进一步检测这个QTL，从用于区间作图的亲本品系之间的回交中获得了一组重组（但非近交）品系。重组品系分析证实，一个QTL由10区带定位。我们鉴定并讨论了我们的QTL区域内包含的候选基因座。
