Gómez Federico H, Defays Raquel, Sambucetti Pablo, Scannapieco Alejandra C, Loeschcke Volker, Norry Fabian M
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Fly (Austin). 2009 Oct-Dec;3(4):247-52. doi: 10.4161/fly.10384. Epub 2009 Oct 21.
Starvation resistance (SR) is an important trait for survival of insects in the wild. We used recombinant inbred lines (RIL) to search for quantitative trait loci (QTL) in crosses between intercontinental inbred lines that were originally selected for heat-knockdown resistance. SR was measured as the time of survival under repeated events of starvation. SR was consistently higher in females than in males. Composite interval mapping identified one QTL region (cytological range 64D-66E2) on the left arm of chromosome 3 in males, and no QTL was found in females. Many candidate genes that were identified in previous studies of QTL for stress resistance are included within this QTL region. The QTL-allele that decreased SR was found in the line originating from the colder population (Denmark). We discuss our results with regard to multiple candidate genes, noncolocalization with thermotolerance QTL, and possible geographical variation.
抗饥饿能力（SR）是昆虫在野外生存的一项重要特性。我们利用重组自交系（RIL）在最初因抗热击倒能力而被选择的洲际自交系之间的杂交中寻找数量性状基因座（QTL）。SR通过在反复饥饿事件下的存活时间来衡量。雌性的SR始终高于雄性。复合区间作图在雄性的3号染色体左臂上鉴定出一个QTL区域（细胞学范围64D - 66E2），而在雌性中未发现QTL。在先前关于抗逆性QTL的研究中鉴定出的许多候选基因都包含在这个QTL区域内。在源自较寒冷种群（丹麦）的品系中发现了降低SR的QTL等位基因。我们就多个候选基因、与耐热性QTL的非共定位以及可能的地理变异对我们的结果进行了讨论。
