Beppu M, Masa H, Hora M, Kikugawa K
Tokyo College of Pharmacy, Japan.
FEBS Lett. 1991 Dec 16;295(1-3):135-40. doi: 10.1016/0014-5793(91)81403-u.
Thioglycollate-induced mouse peritoneal macrophages plated on a coverglass bind oxidized mouse erythrocytes in the absence of serum. Macrophages plated on a coverglass pre-coated with fibronectin (FN) were more active in binding of the oxidized erythrocytes. This effect of FN-coated coverglass was due to specific binding of an RGD-containing sequence of FN to FN-receptors on the macrophage, since GRGDSP hexapeptide in solution inhibited this effect, and GRGDSP-coated coverglass exhibited the same effect as FN-coated coverglass. Removal of FN originally present on the macrophage surface by trypsinization, prior to attachment to the coverglass, resulted in diminution of their ability of recognition of the oxidized erythrocytes, but the diminished ability was restored when the trypsinized macrophages were plated on a FN-coated coverglass, indicating that the cell surface FN is required for the macrophage recognition. Attachment to the coverglass was necessary for the cell surface FN to be effective. These results suggest that solid-phase FN, produced either by deposition of soluble FN to substratum or attachment of macrophage surface FN to substratum, activates the macrophages and augments their ability to recognize the oxidized erythrocytes.
接种在盖玻片上的巯基乙酸盐诱导的小鼠腹腔巨噬细胞在无血清条件下能结合氧化的小鼠红细胞。接种在预先包被纤连蛋白（FN）的盖玻片上的巨噬细胞在结合氧化红细胞方面更活跃。FN包被盖玻片的这种作用是由于FN中含RGD序列与巨噬细胞上的FN受体特异性结合所致，因为溶液中的GRGDSP六肽可抑制这种作用，且GRGDSP包被的盖玻片表现出与FN包被的盖玻片相同的作用。在接种到盖玻片之前，用胰蛋白酶处理去除巨噬细胞表面原本存在的FN，会导致其识别氧化红细胞的能力减弱，但当将经胰蛋白酶处理的巨噬细胞接种在FN包被的盖玻片上时，减弱的能力得以恢复，这表明巨噬细胞识别需要细胞表面FN。细胞表面FN发挥作用需要附着在盖玻片上。这些结果表明，通过将可溶性FN沉积到基质上或巨噬细胞表面FN附着到基质上产生的固相FN可激活巨噬细胞并增强其识别氧化红细胞的能力。
