Raynor R H, Reese A C
Oncology. 1984;41(6):420-6. doi: 10.1159/000225869.
Purified fibronectin (Fn) mediated attachment of MCG-T14 cells, a mouse adenocarcinoma, to culture vessel surfaces. Concentrations of Fn less than 30 micrograms/ml enhanced the growth rate of these cells as judged by 3H-thymidine incorporation, whereas higher levels of Fn were inhibitory. Concentrations of Fn and macrophages, which had little or no effect on the growth rate of the T14 cells when added individually, mediated a 64% decrease in the rate of growth of these cells when cultured together. Free Fn was not required for this effect since target cells pretreated with Fn and then washed also were susceptible to growth inhibition by macrophages. These results indicate that Fn is able to mediate an enhancement of macrophage antitumor activity, probably by supporting the binding of target cells to the macrophage effector cells.
纯化的纤连蛋白（Fn）介导小鼠腺癌MCG-T14细胞附着于培养容器表面。通过³H-胸腺嘧啶核苷掺入法判断，浓度低于30微克/毫升的Fn可提高这些细胞的生长速率，而较高水平的Fn则具有抑制作用。Fn和巨噬细胞的浓度，单独添加时对T14细胞的生长速率几乎没有影响，但共同培养时可介导这些细胞的生长速率下降64%。此效应不需要游离的Fn，因为用Fn预处理后再洗涤的靶细胞也易受巨噬细胞的生长抑制作用影响。这些结果表明，Fn可能通过支持靶细胞与巨噬细胞效应细胞的结合，从而介导巨噬细胞抗肿瘤活性的增强。
