Memoli Bruno, Procino Alfredo, Calabrò Paolo, Esposito Pasquale, Grandaliano Giuseppe, Pertosa Giovanni, Prete Marco Del, Andreucci Michele, Lillo Saverio Di, Ferulano Giuseppe, Cillo Clemente, Savastano Silvia, Colao Annamaria, Guida Brunella
Dept. of Nephrology, Univ. Federico II of Naples, Via S. Pansini 5, 80131, Naples, Italy.
Am J Physiol Endocrinol Metab. 2007 Oct;293(4):E1030-5. doi: 10.1152/ajpendo.00697.2006. Epub 2007 Jul 24.
Only few studies have been addressed to the presence and regulation of C-reactive protein (CRP) gene expression in different districts of adipose tissue, and no study has investigated the role of adipose tissue in presence of inflammation. Therefore, the aim of this study was to investigate the inflammatory involvement of either adipose tissue or adipose cells (adipocytes and stromal cells, respectively) in patients with chronic inflammatory disease, focusing on regional adipose tissue CRP gene expression. Eighteen patients with inflammatory disease and 14 healthy controls were enrolled. All subjects underwent specific surgical procedures. Inflamed and noninflamed patients provided samples of subcutaneous and/or omental adipose tissue. All samples were analyzed by RT-PCR and real-time PCR for specific gene expression. In addition, both adipocytes and stromal cells were studied by real-time PCR and immunoprecipitation to evaluate either gene or protein expression of CRP. Our results (real-time PCR) demonstrated a higher gene expression of CRP, IL-6, and both IL-6 membrane receptors in subcutaneous samples of inflamed patients than in healthy controls. Furthermore, in omental fragments of inflamed patients, an enhanced mRNA abundance of the same genes, compared with subcutaneous, was observed. The results obtained at cellular level did not provide evidence of any difference between adipocytes and stromal cell CRP gene expression, whereas immunoprecipitation demonstrated the presence of CRP in inflamed subjects. These results provide first-time evidence of the involvement of adipose tissue in the course of chronic inflammatory diseases, with a different degree of participation of the different adipose tissue districts.
仅有少数研究探讨了脂肪组织不同区域中C反应蛋白（CRP）基因表达的存在及调控情况，且尚无研究调查炎症状态下脂肪组织的作用。因此，本研究的目的是调查慢性炎症性疾病患者中脂肪组织或脂肪细胞（分别为脂肪细胞和基质细胞）的炎症参与情况，重点关注局部脂肪组织CRP基因的表达。招募了18例炎症性疾病患者和14名健康对照者。所有受试者均接受了特定的外科手术。患有炎症和未患炎症的患者提供了皮下和/或网膜脂肪组织样本。所有样本均通过逆转录聚合酶链反应（RT-PCR）和实时定量PCR分析特定基因的表达。此外，通过实时定量PCR和免疫沉淀法对脂肪细胞和基质细胞进行研究，以评估CRP的基因或蛋白表达。我们的结果（实时定量PCR）表明，与健康对照相比，炎症患者皮下样本中CRP、白细胞介素-6（IL-6）以及两种IL-6膜受体的基因表达更高。此外，与皮下组织相比，在炎症患者的网膜组织碎片中观察到相同基因的信使核糖核酸（mRNA）丰度增加。在细胞水平获得的结果未提供脂肪细胞和基质细胞CRP基因表达存在差异的证据，而免疫沉淀法证明炎症受试者体内存在CRP。这些结果首次证明了脂肪组织参与慢性炎症性疾病的过程，且不同脂肪组织区域的参与程度不同。
