Saillenfait A M, Sabate J P, Langonne I, de Ceaurriz J
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
Fundam Appl Toxicol. 1991 Aug;17(2):399-408. doi: 10.1016/0272-0590(91)90229-w.
Sprague-Dawley rats were administered ethylenethiourea (ETU), 1,3-dimethyl-2-thiourea (DMT), 1,3-dibutyl-2-thiourea (DBT), or 1,3-diphenyl-2-thiourea (DPT) by gavage from Days 6 to 20 of gestation. Daily dosage levels (mg/kg/day) were ETU at 0, 15, 25 and 35; DMT at 0, 15, 25, 50, 100, and 200; DBT at 0, 15, 25, 50, 100, and 200; and DPT at 0, 25, 50, 100, and 200. There was evidence of maternal toxicity at all doses of DMT and at doses greater than or equal to 50 mg DBT/kg/day. DPT was embryolethal at 200 mg/kg/day. Fetotoxicity was observed at doses greater than or equal to 15 mg DMT/kg/day, greater than or equal to 15 mg DBT/kg/day, and greater than or equal to 100 mg DPT/kg/day. ETU was the only chemical tested that proved to be teratogenic.
在妊娠第6天至第20天，通过灌胃法给Sprague-Dawley大鼠施用乙撑硫脲（ETU）、1,3-二甲基-2-硫脲（DMT）、1,3-二丁基-2-硫脲（DBT）或1,3-二苯基-2-硫脲（DPT）。每日剂量水平（毫克/千克/天）分别为：ETU为0、15、25和35；DMT为0、15、25、50、100和200；DBT为0、15、25、50、100和200；DPT为0、25、50、100和200。所有剂量的DMT以及大于或等于50毫克DBT/千克/天的剂量均有母体毒性的证据。DPT在200毫克/千克/天时有胚胎致死性。在大于或等于15毫克DMT/千克/天、大于或等于15毫克DBT/千克/天以及大于或等于100毫克DPT/千克/天的剂量下观察到了胎儿毒性。ETU是所测试的唯一被证明具有致畸性的化学物质。
