Sudomoina M A, Zinovieva O L, Kisselev L L
Engelhardt Institute of Molecular Biology, U.S.S.R. Academy of Sciences, Moscow.
Gene. 1991 Dec 30;109(2):291-6. doi: 10.1016/0378-1119(91)90624-k.
A structural gene encoding bovine (b) tryptophanyl-tRNA synthetase (WRS) has recently been cloned and sequenced [Garret et al., Biochemistry 30 (1991) 7809-7817]. Using part of this sequence as a hybridisation probe we have cloned and sequenced a structural gene encoding human polypeptide highly homologous with two mammalian proteins, bWRS [Garret et al., Biochemistry 30 (1991) 7809-7817; EMBL accession No. X52113] and rabbit peptide chain release factor [Lee et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3508-3512]. Identification of the sequence encoding a human WRS is based on (i) the presence of 'HIGH' and 'KMSKS' structural motifs typical for class-I aminoacyl-tRNA synthetases [Eriani et al., Nature 347 (1990) 203-206]; (ii) coincidence of the number of SH groups per subunit estimated experimentally [Muench et al., Science 187 (1975) 1089-1091] and deduced from the cDNA sequence (six in both cases); (iii) close resemblance of two WRS polypeptides sequenced earlier [Muench et al., Science 187 (1975) 1089-1091] and the predicted structure in two different regions.
最近，一个编码牛（b）色氨酰 - tRNA合成酶（WRS）的结构基因已被克隆并测序[加勒特等人，《生物化学》30（1991）7809 - 7817]。利用该序列的一部分作为杂交探针，我们克隆并测序了一个编码人类多肽的结构基因，该多肽与两种哺乳动物蛋白高度同源，即bWRS[加勒特等人，《生物化学》30（1991）7809 - 7817；欧洲分子生物学实验室登录号X52113]和兔肽链释放因子[李等人，《美国国家科学院院刊》87（1990）3508 - 3512]。人类WRS编码序列的鉴定基于：（i）存在I类氨酰 - tRNA合成酶典型的“HIGH”和“KMSKS”结构基序[埃里亚尼等人，《自然》347（1990）203 - 206]；（ii）实验估计的每个亚基SH基团数量[门奇等人，《科学》187（1975）1089 - 1091]与从cDNA序列推导的数量一致（两种情况下均为六个）；（iii）先前测序的两种WRS多肽[门奇等人，《科学》187（1975）1089 - 1091]与预测结构在两个不同区域的高度相似性。
