• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人类色氨酰-tRNA合成酶通过V85和I311位点的突变转变为一种依赖tRNA的色氨酸激活模式。

Human tryptophanyl-tRNA synthetase is switched to a tRNA-dependent mode for tryptophan activation by mutations at V85 and I311.

作者信息

Guo Li-Tao, Chen Xiang-Long, Zhao Bo-Tao, Shi Yi, Li Wei, Xue Hong, Jin You-Xin

机构信息

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China.

出版信息

Nucleic Acids Res. 2007;35(17):5934-43. doi: 10.1093/nar/gkm633. Epub 2007 Aug 28.

DOI:10.1093/nar/gkm633
PMID:17726052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2034488/
Abstract

For most aminoacyl-tRNA synthetases (aaRS), their cognate tRNA is not obligatory to catalyze amino acid activation, with the exception of four class I (aaRS): arginyl-tRNA synthetase, glutamyl-tRNA synthetase, glutaminyl-tRNA synthetase and class I lysyl-tRNA synthetase. Furthermore, for arginyl-, glutamyl- and glutaminyl-tRNA synthetase, the integrated 3' end of the tRNA is necessary to activate the ATP-PPi exchange reaction. Tryptophanyl-tRNA synthetase is a class I aaRS that catalyzes tryptophan activation in the absence of its cognate tRNA. Here we describe mutations located at the appended beta1-beta2 hairpin and the AIDQ sequence of human tryptophanyl-tRNA synthetase that switch this enzyme to a tRNA-dependent mode in the tryptophan activation step. For some mutant enzymes, ATP-PPi exchange activity was completely lacking in the absence of tRNA(Trp), which could be partially rescued by adding tRNA(Trp), even if it had been oxidized by sodium periodate. Therefore, these mutant enzymes have strong similarity to arginyl-tRNA synthetase, glutaminyl-tRNA synthetase and glutamyl-tRNA synthetase in their mode of amino acid activation. The results suggest that an aaRS that does not normally require tRNA for amino acid activation can be switched to a tRNA-dependent mode.

摘要

对于大多数氨酰-tRNA合成酶（aaRS）而言，其对应的tRNA并非催化氨基酸活化所必需，不过有四种I类aaRS除外：精氨酰-tRNA合成酶、谷氨酰-tRNA合成酶、谷氨酰胺-tRNA合成酶以及I类赖氨酰-tRNA合成酶。此外，对于精氨酰、谷氨酰和谷氨酰胺-tRNA合成酶而言，tRNA完整的3'末端对于激活ATP-PPi交换反应是必需的。色氨酰-tRNA合成酶是一种I类aaRS，在没有其对应tRNA的情况下催化色氨酸的活化。在此，我们描述了位于人色氨酰-tRNA合成酶附加的β1-β2发夹结构和AIDQ序列处的突变，这些突变在色氨酸活化步骤中将该酶转变为依赖tRNA的模式。对于一些突变酶而言，在没有tRNA(Trp)的情况下完全缺乏ATP-PPi交换活性，即使tRNA(Trp)已被高碘酸钠氧化，添加tRNA(Trp)仍可部分挽救这种活性。因此，这些突变酶在氨基酸活化模式上与精氨酰-tRNA合成酶、谷氨酰胺-tRNA合成酶和谷氨酰-tRNA合成酶具有很强的相似性。结果表明，一种通常在氨基酸活化过程中不需要tRNA的aaRS可以转变为依赖tRNA的模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/6c5f126249ca/gkm633f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/50450f21f947/gkm633f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/385bf270da98/gkm633f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/b9a48143ad07/gkm633f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/e58c66bffb18/gkm633f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/bdd89704807c/gkm633f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/6c5f126249ca/gkm633f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/50450f21f947/gkm633f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/385bf270da98/gkm633f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/b9a48143ad07/gkm633f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/e58c66bffb18/gkm633f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/bdd89704807c/gkm633f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee20/2034488/6c5f126249ca/gkm633f6.jpg

相似文献

1
Human tryptophanyl-tRNA synthetase is switched to a tRNA-dependent mode for tryptophan activation by mutations at V85 and I311.人类色氨酰-tRNA合成酶通过V85和I311位点的突变转变为一种依赖tRNA的色氨酸激活模式。
Nucleic Acids Res. 2007;35(17):5934-43. doi: 10.1093/nar/gkm633. Epub 2007 Aug 28.
2
Escherichia coli tryptophanyl-tRNA synthetase mutants selected for tryptophan auxotrophy implicate the dimer interface in optimizing amino acid binding.为色氨酸营养缺陷型筛选出的大肠杆菌色氨酰 - tRNA合成酶突变体表明二聚体界面在优化氨基酸结合方面发挥作用。
Biochemistry. 1996 Jan 9;35(1):32-40. doi: 10.1021/bi952103d.
3
Structure and activity of an aminoacyl-tRNA synthetase that charges tRNA with nitro-tryptophan.一种用硝基色氨酸为tRNA 负载氨基酸的氨酰-tRNA 合成酶的结构与活性
Nat Struct Mol Biol. 2005 Mar;12(3):274-5. doi: 10.1038/nsmb907. Epub 2005 Feb 20.
4
Tryptophanyl-tRNA synthetase from Bacillus subtilis. Characterization and role of hydrophobicity in substrate recognition.来自枯草芽孢杆菌的色氨酰-tRNA合成酶。疏水性在底物识别中的特性及作用
J Biol Chem. 1989 Mar 15;264(8):4304-11.
5
Role of the TIGN sequence in E. coli tryptophanyl-tRNA synthetase.TIGN序列在大肠杆菌色氨酰-tRNA合成酶中的作用。
Biochim Biophys Acta. 1994 Apr 13;1205(2):223-9. doi: 10.1016/0167-4838(94)90237-2.
6
Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity.与色氨酸转运RNA（tRNATrp）结合的人色氨酰-tRNA合成酶结构揭示了tRNA识别和特异性的分子基础。
Nucleic Acids Res. 2006 Jun 23;34(11):3246-58. doi: 10.1093/nar/gkl441. Print 2006.
7
Reaction of tryptophanyl-tRNA synthetase from beef pancreas with periodate-oxidized ATP.牛胰色氨酰 - tRNA合成酶与高碘酸盐氧化的ATP的反应。
Eur J Biochem. 1987 Sep 15;167(3):533-40. doi: 10.1111/j.1432-1033.1987.tb13370.x.
8
Substrate selection by aminoacyl-tRNA synthetases.氨酰-tRNA合成酶对底物的选择
Nucleic Acids Symp Ser. 1995(33):40-2.
9
Two essential regions for tRNA recognition in Bacillus subtilis tryptophanyl-tRNA synthetase.枯草芽孢杆菌色氨酰-tRNA合成酶中识别tRNA的两个关键区域。
Biochem J. 2002 Aug 1;365(Pt 3):749-56. doi: 10.1042/BJ20020141.
10
Docking of tryptophanyl [corrected tryptophan] analogs to trytophanyl-tRNA synthetase: implications for non-canonical amino acid incorporations.色氨酰[校正为色氨酸]类似物与色氨酰-tRNA合成酶的对接：对非标准氨基酸掺入的影响。
Biol Chem. 2008 Sep;389(9):1173-82. doi: 10.1515/BC.2008.133.

引用本文的文献

1
The PERK Branch of the Unfolded Protein Response Safeguards Protein Homeostasis and Mesendoderm Specification of Human Pluripotent Stem Cells.未折叠蛋白反应的 PERK 分支保护人多能干细胞的蛋白质动态平衡和中胚层特化。
Adv Sci (Weinh). 2023 Dec;10(35):e2303799. doi: 10.1002/advs.202303799. Epub 2023 Oct 27.
2
Backbone Brackets and Arginine Tweezers delineate Class I and Class II aminoacyl tRNA synthetases.骨干支架和精氨酸镊子区分 I 类和 II 类氨酰基 tRNA 合成酶。
PLoS Comput Biol. 2018 Apr 16;14(4):e1006101. doi: 10.1371/journal.pcbi.1006101. eCollection 2018 Apr.
3
Rational design and directed evolution of a bacterial-type glutaminyl-tRNA synthetase precursor.

本文引用的文献

1
A minimal TrpRS catalytic domain supports sense/antisense ancestry of class I and II aminoacyl-tRNA synthetases.一个最小的色氨酰-tRNA合成酶催化结构域支持I类和II类氨酰-tRNA合成酶的有义/反义起源。
Mol Cell. 2007 Mar 23;25(6):851-62. doi: 10.1016/j.molcel.2007.02.010.
2
Structural bases of transfer RNA-dependent amino acid recognition and activation by glutamyl-tRNA synthetase.谷氨酰胺-tRNA合成酶依赖转运RNA的氨基酸识别与激活的结构基础
Structure. 2006 Dec;14(12):1791-9. doi: 10.1016/j.str.2006.10.005.
3
Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity.
理性设计和定向进化细菌型谷氨酰-tRNA 合成酶前体。
Nucleic Acids Res. 2012 Sep;40(16):7967-74. doi: 10.1093/nar/gks507. Epub 2012 May 31.
4
Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase.色氨酰-tRNA合成酶的三种原生动物同源物的晶体结构。
Mol Biochem Parasitol. 2011 May;177(1):20-8. doi: 10.1016/j.molbiopara.2011.01.003. Epub 2011 Jan 19.
5
The structure of tryptophanyl-tRNA synthetase from Giardia lamblia reveals divergence from eukaryotic homologs.蓝氏贾第鞭毛虫色氨酰-tRNA 合成酶的结构揭示了与真核同源物的差异。
J Struct Biol. 2010 Aug;171(2):238-43. doi: 10.1016/j.jsb.2010.04.010. Epub 2010 May 8.
6
Catalytic mechanism of the tryptophan activation reaction revealed by crystal structures of human tryptophanyl-tRNA synthetase in different enzymatic states.不同酶促状态下人色氨酰 - tRNA合成酶晶体结构揭示的色氨酸激活反应催化机制
Nucleic Acids Res. 2008 Mar;36(4):1288-99. doi: 10.1093/nar/gkm1153. Epub 2008 Jan 7.
与色氨酸转运RNA（tRNATrp）结合的人色氨酰-tRNA合成酶结构揭示了tRNA识别和特异性的分子基础。
Nucleic Acids Res. 2006 Jun 23;34(11):3246-58. doi: 10.1093/nar/gkl441. Print 2006.
4
Two conformations of a crystalline human tRNA synthetase-tRNA complex: implications for protein synthesis.一种结晶态的人类氨酰-tRNA合成酶-tRNA复合物的两种构象：对蛋白质合成的影响。
EMBO J. 2006 Jun 21;25(12):2919-29. doi: 10.1038/sj.emboj.7601154. Epub 2006 May 25.
5
Determinants in tRNA for activation of arginyl-tRNA synthetase: evidence that tRNA flexibility is required for the induced-fit mechanism.tRNA中精氨酰-tRNA合成酶激活的决定因素：tRNA灵活性是诱导契合机制所必需的证据。
Biochemistry. 2005 Dec 20;44(50):16540-8. doi: 10.1021/bi051575h.
6
Domain-domain communication for tRNA aminoacylation: the importance of covalent connectivity.用于tRNA氨基酰化的结构域间通讯：共价连接的重要性。
Biochemistry. 2005 May 17;44(19):7240-9. doi: 10.1021/bi050285y.
7
In vitro characterization of a tRNA editing activity in the mitochondria of Spizellomyces punctatus, a Chytridiomycete fungus.对壶菌纲真菌点状梭孢酵母线粒体中一种tRNA编辑活性的体外特性研究。
J Biol Chem. 2005 Jan 28;280(4):2463-70. doi: 10.1074/jbc.M411273200. Epub 2004 Nov 15.
8
Residues Lys-149 and Glu-153 switch the aminoacylation of tRNA(Trp) in Bacillus subtilis.赖氨酸-149和谷氨酸-153残基改变了枯草芽孢杆菌中tRNA(Trp)的氨酰化作用。
J Biol Chem. 2004 Oct 1;279(40):41960-5. doi: 10.1074/jbc.M401937200. Epub 2004 Jul 26.
9
Crystal structures that suggest late development of genetic code components for differentiating aromatic side chains.晶体结构表明用于区分芳香族侧链的遗传密码组件的后期发展。
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15376-80. doi: 10.1073/pnas.2136794100. Epub 2003 Dec 11.
10
GLUTAMYL AND GLUTAMINYL RIBONUCLEIC ACID SYNTHETASES OF ESCHERICHIA COLI W. SEPARATION, PROPERTIES, AND STIMULATION OF ADENOSINE TRIPHOSPHATE-PYROPHOSPHATE EXCHANGE BY ACCEPTOR RIBONUCLEIC ACID.大肠杆菌W的谷氨酰胺和谷氨酰核糖核酸合成酶。分离、特性以及受体核糖核酸对三磷酸腺苷-焦磷酸交换的刺激作用
J Biol Chem. 1965 Jan;240:432-8.