Lei Chun-liang, Huang Cheng-hui, Yang Zhan, Tang Xiao-ping
The Eighth Hospital of Guangzhou City, Guangzhou 510060, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Jun;21(2):108-10.
To explore whether hepatitis B virus (HBV) S gene-modified dendritic cells (DCs) might induce a specific cytotoxic T lymphocyte (CTL) response.
The recombinant adenoviruses carrying HBsAg genes were prepared and used to transfect DCs generated from cord blood. The efficacy of transfection was observed through the expression of enhanced green fluorescent protein (EGFP) in DCs and the expression of HBsAg was detected by ELISA. HBV S gene-modified DCs were co-cultured with T cells from cord blood and T cells stimulating activities were detected using mixed lymphocyte reaction (MLR). The CTL assay was carried out to assess the ability of CTL lines to lyse target cells of HepG(2)22.1.5 by measuring lactate dehydrogenase (LDH) release.
The results showed that HBV S genes were expressed in DCs with high efficacy by recombinant adenoviral vector. DCs had a normal shape after transfection. The result of MLR showed that HBV S gene-modified DCs could effectively stimulate naive T cells to proliferate. The induced specific CTL lines could lyse target cells of HepG(2)22.1.5.
HBV S gene-modified DCs enhanced the function to induce a specific CTL effect, showing its promise for developing anti-viral vaccine in future.
探讨乙型肝炎病毒（HBV）S基因修饰的树突状细胞（DCs）是否能诱导特异性细胞毒性T淋巴细胞（CTL）反应。
制备携带HBsAg基因的重组腺病毒，用于转染脐血来源的DCs。通过DCs中增强绿色荧光蛋白（EGFP）的表达观察转染效率，采用酶联免疫吸附测定（ELISA）检测HBsAg的表达。将HBV S基因修饰的DCs与脐血T细胞共培养，采用混合淋巴细胞反应（MLR）检测T细胞刺激活性。通过检测乳酸脱氢酶（LDH）释放，进行CTL检测以评估CTL系裂解HepG(2)22.1.5靶细胞的能力。
结果显示重组腺病毒载体能使HBV S基因在DCs中高效表达。转染后DCs形态正常。MLR结果表明，HBV S基因修饰的DCs能有效刺激初始T细胞增殖。诱导产生的特异性CTL系能裂解HepG(2)22.1.5靶细胞。
HBV S基因修饰的DCs增强了诱导特异性CTL效应的功能，显示出其在未来开发抗病毒疫苗方面的前景。
