Fan Chun-lei, Chen Hong-song, Li Ruo-bing, Wang Song-xia, Cong Xu, Fei Ran, Jiang Dong, Wang Yu, Wei Lai
Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Zhonghua Gan Zang Bing Za Zhi. 2004 Feb;12(2):67-71.
To investigate the correlation between impaired non-viral specific immune function of dendritic cell (DC) and viral clearance and cytotoxic T lymphocyte (CTL) response to HBV or HCV in patients with HBV and HCV coinfection.
Twenty-five patients with HBV and HCV coinfection were investigated in this study. In 1994 and 2002, biochemical and virological markers and quantitative serum HBV DNA and HCV RNA levels were detected in these patients. According to the virus clearance status, these patients were divided into 4 groups: 14 patients with both HBV and HCV clearance (Group A), 6 patients with HCV clearance only (Group B), 3 patients with HBV clearance only (Group C), and 2 patients with persistent infection of HBV and HCV (Group D). Phenotypes and immune functions of monocyte-derived DCs were compared between these groups. 51Cr release assay were used to measure CTL response to epitopes derived from HBV, HCV or influenza virus (as positive control) in HLA-A2+ patients.
Impaired non-viral specific immune functions of DCs were observed in group B, C and D compared with group A and normal donors (Group N). These impaired functions included CD86 decreasing expression and lower capacity to stimulating allogenic T cells and uptaking antigen. The specific CTL response to HBV- and HCV-derived peptides could be induced in group A (12/12). The specific CTL response to HBV-derived peptides or to HCV-derived peptides could be induced in group C (3/3) or B (5/5), respectively. But the specific CTL response to both of two HBV-derived peptides or two HCV-derived peptides could not be induced in group C (0/3) or B (0/5), respectively. And no CTL response to HBV or HCV-derived peptides could be induced in groups D (0/1) and N (0/4).
探讨乙肝病毒（HBV）和丙肝病毒（HCV）合并感染患者中树突状细胞（DC）非病毒特异性免疫功能受损与病毒清除以及细胞毒性T淋巴细胞（CTL）对HBV或HCV反应之间的相关性。
本研究纳入了25例HBV和HCV合并感染患者。在1994年和2002年，检测了这些患者的生化和病毒学标志物以及血清HBV DNA和HCV RNA定量水平。根据病毒清除状态，将这些患者分为4组：14例HBV和HCV均清除的患者（A组），6例仅HCV清除的患者（B组），3例仅HBV清除的患者（C组），以及2例HBV和HCV持续感染的患者（D组）。比较了这些组之间单核细胞来源的DC的表型和免疫功能。采用51Cr释放试验检测HLA-A2+患者对来自HBV、HCV或流感病毒（作为阳性对照）表位的CTL反应。
与A组和正常供体（N组）相比，B组、C组和D组观察到DC的非病毒特异性免疫功能受损。这些受损功能包括CD86表达降低以及刺激同种异体T细胞和摄取抗原的能力降低。A组（12/12）可诱导对HBV和HCV来源肽的特异性CTL反应。C组（3/3）或B组（5/5）分别可诱导对HBV来源肽或HCV来源肽的特异性CTL反应。但C组（0/3）或B组（0/5）分别不能诱导对两种HBV来源肽或两种HCV来源肽的特异性CTL反应。D组（0/1）和N组（0/4）对HBV或HCV来源肽均无CTL反应。
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