Mercer Andrew C, Burkart Michael D
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, USA.
Nat Prod Rep. 2007 Aug;24(4):750-73. doi: 10.1039/b603921a. Epub 2007 Apr 3.
Nature has developed a remarkable strategy to isolate metabolites from the milieu of the cell for chemical modification through the use of carrier proteins. Common to both primary and secondary metabolic pathways, acyl-carrier proteins constitute a conserved protein architecture which mediate the biosynthesis of a variety of metabolic products. Analogies have been made between the carrier protein and solid phase resin for chemical synthesis, as both entities provide a mechanism to separate compounds of interest from complex mixtures for selective chemical modification. However, there is significantly more to the carrier protein than an attachment point. In this review, we aim to systematically characterize the role of carrier proteins in various metabolic pathways and outline their utility in biosynthesis and biotechnology; 185 references are cited.
大自然已经发展出一种非凡的策略，通过使用载体蛋白将代谢物与细胞环境隔离开来进行化学修饰。酰基载体蛋白是初级和次级代谢途径共有的，构成了一种保守的蛋白质结构，介导多种代谢产物的生物合成。载体蛋白与用于化学合成的固相树脂之间存在相似之处，因为这两种实体都提供了一种从复杂混合物中分离目标化合物以进行选择性化学修饰的机制。然而，载体蛋白的作用远不止是一个附着点。在这篇综述中，我们旨在系统地描述载体蛋白在各种代谢途径中的作用，并概述它们在生物合成和生物技术中的应用；引用了185篇参考文献。
