通过对一例肿瘤快速进展的少突胶质细胞瘤进行蛋白质组学研究，探讨与少突胶质细胞瘤恶性转化相关的分子因素。
Investigation of molecular factors associated with malignant transformation of oligodendroglioma by proteomic study of a single case of rapid tumor progression.
作者信息
Park Chul-Kee, Kim Jin Hyun, Moon Min Jeong, Jung Ji Hye, Lim Su-Young, Park Sung-Hye, Kim Jong-Hoon, Kim Dong Gyu, Jung Hee-Won, Cho Byung-Kyu, Paek Sun Ha
机构信息
Department of Neurosurgery, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, South Korea.
出版信息
J Cancer Res Clin Oncol. 2008 Feb;134(2):255-62. doi: 10.1007/s00432-007-0282-1. Epub 2007 Jul 26.
Abstract
PURPOSE
Frozen tumor tissues from a patient who showed rapid progression to anaplastic oligodendroglioma after near total resection of oligodendroglioma were used to examine differential expression of proteins to gain better understanding of the pathogenesis of malignant transformation.
METHODS
We have determined their protein profiles using a 2D gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry approach.
RESULTS
Among 23 differentially expressed spots, overexpression of peroxiredoxin 6 and underexpression of rho GDP dissociation inhibitor alpha were confirmed to be valid after western blot and immunocytochemical analysis of oligodendroglioma tissue.
CONCLUSIONS
Abnormal expression of peroxiredoxin 6 and rho GDP dissociation inhibitor alpha may be associated with malignant transformation in oligodendroglioma and these proteins might be candidates of molecular predictive factors.
摘要
目的
使用来自一名少突胶质细胞瘤近全切术后迅速进展为间变性少突胶质细胞瘤患者的冷冻肿瘤组织，检测蛋白质的差异表达，以更好地了解恶性转化的发病机制。
方法
我们采用二维凝胶电泳和基质辅助激光解吸/电离飞行时间质谱方法确定了它们的蛋白质谱。
结果
在23个差异表达斑点中，通过对少突胶质细胞瘤组织进行蛋白质印迹和免疫细胞化学分析，证实过氧化物还原酶6的过表达和rho GDP解离抑制剂α的低表达是有效的。
结论
过氧化物还原酶6和rho GDP解离抑制剂α的异常表达可能与少突胶质细胞瘤的恶性转化有关，这些蛋白质可能是分子预测因子的候选者。
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