Nell Sandra, Bahtz Ramona, Bossecker Anja, Kipp Anna, Landes Nico, Bumke-Vogt Christiane, Halligan Eugene, Lunec Joseph, Brigelius-Flohé Regina
German Institute of Human Nutrition Potsdam-Rehbrücke, Nuthetal, Germany.
Free Radic Res. 2007 Aug;41(8):930-42. doi: 10.1080/10715760701416988.
Global gene expression profiles of livers from mice, fed diets differing in alpha-tocopherol content, were compared using DNA microarray technology. Three hundred and eighty nine genes were found to significantly differ in their expression level by a factor of 2 or higher between the high and the low alpha-tocopherol group. Functional clustering using the EASE software identified 121 genes involved in transport processes. Twenty-one thereof were involved in (synaptic) vesicular trafficking. Up-regulation of syntaxin 1C (Stx1c), vesicle-associated membrane protein 1 (Vamp1), N-ethylmaleimide-sensitive factor (Nsf) and syntaxin binding protein 1 (Stxbp1, Munc18-1) was verified by real time PCR. At a functional level, alpha-tocopherol increased the secretory response in RBL and PC12 cells. Although here detected in liver, the alpha-tocopherol-responsive pathways are also relevant to neurotransmission. A role of alpha-tocopherol in the vesicular transport might not only affect its own absorption and transport but also explain the neural dysfunctions observed in severe alpha-tocopherol deficiency.
利用DNA微阵列技术比较了喂食不同α-生育酚含量饮食的小鼠肝脏的全球基因表达谱。发现389个基因在高α-生育酚组和低α-生育酚组之间的表达水平有显著差异，差异因子为2或更高。使用EASE软件进行功能聚类，鉴定出121个参与转运过程的基因。其中21个参与（突触）囊泡运输。通过实时PCR验证了 syntaxin 1C（Stx1c）、囊泡相关膜蛋白1（Vamp1）、N-乙基马来酰亚胺敏感因子（Nsf）和 syntaxin 结合蛋白1（Stxbp1，Munc18-1）的上调。在功能水平上，α-生育酚增加了RBL和PC12细胞的分泌反应。尽管在肝脏中检测到，但α-生育酚反应途径也与神经传递有关。α-生育酚在囊泡运输中的作用可能不仅影响其自身的吸收和运输，还可以解释在严重α-生育酚缺乏时观察到的神经功能障碍。
