12C6+离子辐照诱导小鼠免疫功能的改变。

Alterations of immune functions induced by 12C6+ ion irradiation in mice.

作者信息

Xie Y, Zhang H, Wang Y L, Zhou Q M, Qiu R, Yuan Z G, Zhou G M

机构信息

Department of Radiology, Institute of Modern Physics, the Chinese Academy of Science, Lanzhou, Gansu Province, PR China.

出版信息

Int J Radiat Biol. 2007 Sep;83(9):577-81. doi: 10.1080/09553000701481774.

DOI:10.1080/09553000701481774

PMID:17654099

Abstract

PURPOSE

To estimate the biological risks to the immune system of the type of space radiation, 12C6+, encountered by cosmonauts during long-term travel in space.

MATERIALS AND METHODS

The Kun-Ming strain mice were whole-body irradiated by 12C6+ ion with 0, 0.01, 0.05, 0.075, 0.2, 0.3, 0.5, 0.75, 1 or 2 Gy, at a dose rate of 1 Gy/min. At 35 days after irradiation, the thymus and spleen weights were measured, the natural killer (NK) cells activity of spleen was determined by 3-(4, 5-dimethylthiazol-2-yl)- 2, 5-diphenyl tetrazolium bromide (MTT), and the interferon-gamma (IFN-gamma) levels in serum and thymus were detected with enzyme-linked immunosorbent assays (ELISA).

RESULTS

The results showed that the thymus weight, IFN-gamma levels in serum and the activity of splenic NK-cells had significantly increased at a dose of 0.05 Gy. With further dose increase, the weight of spleen continued to increase but the weight of thymus, IFN-gamma level and NK-cells activity declined.

CONCLUSIONS

These results suggest that the dose of 0.05 Gy irradiation has a stimulatory effect on mouse immunity; this effect declined with increasing dose.

摘要

目的

评估宇航员在太空长期旅行期间所遭遇的12C6+这种空间辐射类型对免疫系统的生物学风险。

材料与方法

将昆明种小鼠以1 Gy/min的剂量率，分别用0、0.01、0.05、0.075、0.2、0.3、0.5、0.75、1或2 Gy的12C6+离子进行全身照射。照射后35天，测量胸腺和脾脏重量，用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐(MTT)法测定脾脏自然杀伤(NK)细胞活性，并用酶联免疫吸附测定(ELISA)法检测血清和胸腺中γ干扰素(IFN-γ)水平。