Zhu Bin, Bai Gui-Chang, Jiang Shou-Jun, Wei Feng, Lin Rui-Chao
Guangxi Institute for Food and Drug Control, Nanning 530021, China.
Zhongguo Zhong Yao Za Zhi. 2007 May;32(10):932-4.
To investigate the chemical constituents and establish a quantitative method of Mallotus apelta.
Compound was isolated by silica gel, Sephadex LH-20 column chromatography and Pre-HPLC chromatography. Its structure was identified by physicochemical properties and spectral evidences. The content of M. apelta was determined by HPLC. Chromatographic conditions included Inertsil ODS-3 C18 column (4.6 mm x 150 mm, 5 microm) and the mobile phase consisting of a mixture of methanol-water (24:76). The detection wavelength was set at 335 nm.
One compound was isolated from n-butanol extract of the M. apelta and its structure was identified as vicenin II. The calibration cure was linear in the range of 0.053-10.60 microg (r = 0.9999), the average recovery was 99. 32%, RSD 1.82% (n = 6).
The compound was isolated from this plant for the first time. The method to determine the content of vicenin II by HPLC was established for the first time. This method is simple, accurate and reliable.
研究山麻杆的化学成分并建立其定量分析方法。
采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱及制备型高效液相色谱法分离化合物。通过理化性质和光谱数据鉴定其结构。采用高效液相色谱法测定山麻杆中目标成分的含量。色谱条件为：Inertsil ODS-3 C18柱（4.6 mm×150 mm，5 μm），流动相为甲醇-水（24∶76）混合溶液，检测波长为335 nm。
从山麻杆正丁醇提取物中分离得到1个化合物，鉴定为异荭草苷。其线性范围为0.053～10.60 μg（r = 0.9999），平均回收率为99.32%，相对标准偏差为1.82%（n = 6）。
该化合物首次从该植物中分离得到。首次建立了高效液相色谱法测定异荭草苷含量的方法。该方法简便、准确、可靠。
