[采用反相高效液相色谱法测定白花丹不同部位的白花丹醌]
[Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC].
作者信息
Liu Yuan, Deng Fang, Liu Chao, Meng Qing-Yan, Gao Ze-Wen
机构信息
Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Oct;31(20):1684-6.
OBJECTIVE
To develop a RP-HPLC method to determine plumbagin in Plumbago zeylanica, and to investigate contents of plumbagin in different parts of. P. zeylanica.
METHOD
The analysis was carried out at 30 degrees C on a Kromasil C18, column eluted with a mobile phase consisting of a mixture of methanol-water (65: 35). The flow rate was 1 mL x min(-1), the detector wavelength was 213 nm.
RESULT
The calibration curve was linear within the concentration ranges of 0.020 8-0. 104 microg (r = 0. 9999). The average recovery was 98.7%. The contents in the root, stem and leaf were 0.394 5%, 0.050 8%, 0.031 4% respectively.
CONCLUSION
This method is simple, accurate, replicate and suitable for the determination of plumbagin in P. zeylanica.
目的
建立反相高效液相色谱法测定白花丹中白花丹醌的含量，并考察白花丹不同部位中白花丹醌的含量。
方法
采用Kromasil C18柱，在30℃下进行分析，流动相为甲醇 - 水（65∶35）混合溶液，流速为1 mL·min⁻¹，检测波长为213 nm。
结果
在0.020 8 - 0.104 μg浓度范围内，校正曲线呈线性（r = 0.9999），平均回收率为98.7%。根、茎、叶中含量分别为0.394 5%、0.050 8%、0.031 4%。
结论
该方法简便、准确、重现性好，适用于白花丹中白花丹醌的含量测定。
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