Wutticharoenmongkol Patcharaporn, Pavasant Prasit, Supaphol Pitt
Technological Center for Electrospun Fibers and The Petroleum and Petrochemical College and Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
Biomacromolecules. 2007 Aug;8(8):2602-10. doi: 10.1021/bm700451p. Epub 2007 Jul 27.
Electrospun (e-spun) fiber mats of polycaprolactone (PCL; Mn = 80 000 g mol-1) with or without the presence of hydroxyapatite (HAp) nanoparticles (at 1% w/v based on the volume of the PCL solution) were successfully fabricated. The potential for use of these e-spun fiber mats as bone scaffolds was assessed by mouse calvaria-derived pre-osteoblastic cells, MC3T3-E1, in terms of attachment, proliferation, differentiation, and mineralization. Despite the lower number of cells attached at early time points, both the fibrous scaffolds supported the proliferation of MC3T3-E1 at similar levels to tissue-culture polystyrene plate (TCPS), with the cells growing on the PCL/HAp fiber mat (i.e., PCL/HAp-FS) showing the greatest proliferation rate on day 3 after the initial attachment period of 16 h. Alkaline phosphatase (ALP) activity of the cells grown on TCPS was the greatest on day 3 after cell culturing, while that of the cells grown on PCL/HAp-FS reached a maximum on day 5. On the other hand, the ALP activity of the cells grown on the neat PCL fiber mat (i.e., PCL-FS) was the lowest at any given time point. MC3T3-E1 cultured on the surface of PCL/HAp-FS expressed the greatest amount of osteocalcin (OC) gene on day 14 after cell culturing and OC protein on day 21 after cell culturing, respectively, when compared with those cultured on the surfaces of PCL-FS and TCPS. This corresponded to the greatest extent of mineralization for the cells grown on the surface of PCL/HAp-FS on day 21, followed by that for the cells grown on PCL-FS and TCPS, respectively.
成功制备了含有或不含羟基磷灰石（HAp）纳米颗粒（基于聚己内酯（PCL；Mn = 80 000 g mol-1）溶液体积，含量为1% w/v）的电纺（e-spun）聚己内酯纤维垫。通过小鼠颅骨来源的前成骨细胞MC3T3-E1，从细胞附着、增殖、分化和矿化方面评估了这些电纺纤维垫作为骨支架的应用潜力。尽管在早期时间点附着的细胞数量较少，但两种纤维支架都能以与组织培养聚苯乙烯板（TCPS）相似的水平支持MC3T3-E1的增殖，在最初16小时的附着期后第3天，生长在PCL/HAp纤维垫（即PCL/HAp-FS）上的细胞显示出最大的增殖率。在TCPS上生长的细胞的碱性磷酸酶（ALP）活性在细胞培养后第3天最大，而在PCL/HAp-FS上生长的细胞的ALP活性在第5天达到最大值。另一方面，在纯PCL纤维垫（即PCL-FS）上生长的细胞的ALP活性在任何给定时间点都是最低的。与在PCL-FS和TCPS表面培养的细胞相比，在PCL/HAp-FS表面培养的MC3T3-E1在细胞培养后第14天分别表达了最大量的骨钙素（OC）基因，在细胞培养后第21天表达了最大量的OC蛋白。这对应于在第21天在PCL/HAp-FS表面生长的细胞的矿化程度最大，其次分别是在PCL-FS和TCPS上生长的细胞。
